何亮娜 马俊美 范力欣 李强

摘 要：建立畜禽肉及肉制品中2 种儿茶酚胺类化合物（肾上腺素和多巴胺）的超高效液相色谱-质谱联用检测方法。样品采用10 mmol/L磷酸二氢钾溶液提取，WCX固相萃取柱净化，Waters Acquity BEH C18柱分离，采用电喷雾离子源，正离子多反应模式监测，基质匹配内标法定量。结果表明：肾上腺素和多巴胺在0.5～50 ng/mL范围内，相关系数（R2）均大于0.996;在畜肉中的检出限和定量限分别为0.39～0.40 μg/kg和1.00 μg/kg，在禽肉中的检出限和定量限分别为0.36～0.37 μg/kg和0.98 μg/kg，在肉制品中的检出限和定量限分别为0.38 μg/kg和0.96～0.97 μg/kg;加标量为1～10 μg/kg时，方法回收率为83.7%～111.2%，相对标准偏差为1.28%～5.76%。该方法具有灵敏度高、定量准确等优点，适用于畜禽肉及肉制品中残留儿茶酚胺类化合物含量的测定。

关键词：固相萃取;超高效液相色谱-质谱法;畜禽肉及肉制品;儿茶酚胺

Determination of Epinephrine and Dopamine Residues in Meat and Meat Products by Solid Phase Extraction Combined with Ultra-High Performance Liquid Chromatography-Mass Spectrometry

HE Liangna， MA Junmei， FAN Lixin， LI Qiang*

（Hebei Food Safety Key Laboratory， Hebei Food Inspection and Research Institute， Shijiazhuang 050299， China）

Abstract： An ultra-high performance liquid chromatography-mass spectrometry （UPLC-MS） method was established for the detection of the residues of two catecholamine compounds （epinephrine and dopamine） in meat and meat products. The samples were extracted with 10 mmol/L phosphate buffer solution， and the extract was purified on a WCX solid phase extraction column， and separated on a Waters Acquity BEH C18 column. The analytes were detected using an electrospray ion source in the positive ion multiple reaction monitoring （MRM） mode， and quantified by a matrix matched internal standard method. The results showed that the calibration curves for epinephrine and dopamine were linear in the concentration range of 0.5–50 ng/mL with correlation coefficient （R2） greater than 0.996. The limits of detection and limits of quantification were 0.39–0.40 and 1.00 μg/kg for livestock meat， 0.36–0.37 and 0.98 μg/kg for poultry meat， and 0.38 and 0.96–0.97 μg/kg for meat products， respectively. At spiked concentrations of 1–10 μg/kg， the recoveries of the method were between 83.7% and 111.2%， and the precision relative standard deviation （RSD） was 1.28%–5.76%. This method has the advantages of high sensitivity and accuracy， and is suitable for the determination of residual catecholamine compounds in meat and meat products.

Keywords： solid phase extraction; ultra-high performance liquid chromatography-mass spectrometry; meat and meat products; catecholamine

DOI：10.7506/rlyj1001-8123-20210422-107

中圖分类号：TS251.5                                       文献标志码：A 文章编号：1001-8123（2021）09-0027-06

引文格式：

何亮娜， 马俊美， 范力欣， 等. 固相萃取结合超高效液相色谱-质谱法测定畜禽肉及肉制品中肾上腺素和多巴胺的残留量[J]. 肉类研究， 2021， 35（9）： 27-32. DOI：10.7506/rlyj1001-8123-20210422-107.    http：//www.rlyj.net.cn

HE Liangna， MA Junmei， FAN Lixin， et al. Determination of epinephrine and dopamine residues in meat and meat products by solid phase extraction combined with ultra-high performance liquid chromatography-mass spectrometry[J]. Meat Research， 2021， 35（9）： 27-32. DOI：10.7506/rlyj1001-8123-20210422-107.    http：//www.rlyj.net.cn

肾上腺素（epinephrine，EP）和多巴胺（dopamine，DM）属于儿茶酚胺类物质，在生物体内具有增强心肌收缩力和增加血流量的作用[1-2]。EP和DM的结构式如图1所示。这2 种物质在维持机体生理平衡中起着至关重要的作用，并且其体内水平与一些病理现象息息相关[3-6]。此外，EP还具有促进动物生长的作用[7]。然而，越来越多的不法商贩为取得暴利，强行给待宰的畜禽注射儿茶酚胺类药物，以提高畜禽肉中的水分含量[8-10]。

经过该方式进行注水的畜禽肉会呈现颜色鲜红的特点，卖相较好[10]。……