汤样华 杨振杰 莫亚峰 任国华 侯桥 辛大伟 曾林如

[摘要] 目的 觀察益肾除痹方对类风湿关节炎大鼠血清及关节滑膜细胞中炎症因子TNF-α、IL-1β、INF-γ的影响。 方法 选择24只类风湿关节炎大鼠随机分为模型组、阳性药物对照组、益肾除痹方低剂量组、益肾除痹方高剂量组，每组各6只，另取6只大鼠为空白组（正常组）。药物连续干预4周，于第2、4周，取大鼠血清及进行关节滑膜细胞培养，采用ELISA法检测大鼠血清及关节滑膜细胞中TNF-α、IL-1β、INF-γ的含量。 结果 模型组大鼠血清及滑膜细胞中的TNF-α、IL-1β、IFN-γ含量均高于空白组（P<0.05）。阳性药物对照组和益肾除痹方高剂量组大鼠血清及滑膜细胞中的TNF-α、IL-1β、IFN-γ含量均明显低于模型组（P<0.05）;而益肾除痹方低剂量组中除第2周所取血清中的TNF-α、IFN-γ和滑膜细胞中的TNF-α、IL-1β与模型组差异无统计学意义外，其余均明显低于模型组（P<0.05）。 结论 益肾除痹方治疗类风湿关节炎的作用机制与降低炎症因子TNF-α、IL-1β、IFN-γ的水平有关。

[关键词] 益肾除痹方;类风湿关节炎;炎症因子;作用机制

[中图分类号] R245.81;R593.21          [文献标识码] A  &nbsp;       [文章编号] 1673-9701（2021）35-0027-05

Impacts of Yishen Chubi Prescription on inflammatory factors TNF-α， IL-1β and INF-γ in serum and joint synovial cells of rats with rheumatoid arthritis

TANG Yanghua   YANG Zhenjie   MO Yafeng   REN Guohua   HOU Qiao   XIN Dawei   ZENG Linru

Department of Orthopedics， Xiaoshan Hospital of Traditional Chinese Medicine in Hangzhou， Jiangnan Hospital Affiliated to Zhejiang Chinese Medical University， Hangzhou   311201， China

[Abstract] Objective To observe the impacts of Yishen Chubi Prescription on inflammatory factors TNF-α， IL-1β and INF-γ in serum and joint synovial cells of rats with rheumatoid arthritis. Methods A total of 24 rats with rheumatoid arthritis were randomly divided into the model group（n=6）， the positive drug control group （n=6）， the low-dose Yishen Chubi prescription group（n=6） and the high-dose Yishen Chubi Prescription group（n=6）. Other rats were selected as the blank group （the normal group， n=6）. Drug intervention continued for 4 weeks. In the second and fourth week， rat serum and synovial cells were collected and cultured. And the contents of TNF-α， IL-1β and INF-γ in rat serum and synovial cells were detected by ELISA method. Results The contents of TNF-α， IL-1β and IFN-γ in rat serum and synovial cells of the model group were all higher than those of the blank group（P<0.05）. The contents of TNF-α， IL-1β and IFN-γ in rat serum and synovial cells in the positive drug control group and the high-dose Yishen Chubi prescription group were all significantly lower than those in the model group（P<0.05）. However， there were no statistically significant differences between TNF-α， IFN-γ in serum and TNF-α， IL-1β in synovial cells of the second week in the low-dose Yishen Chubi prescription group and those in the model group. Except for this， the rest indices were all significantly lower than those in the model group（P<0.05）. Conclusion The mechanism of function of Yishen Chubi Prescription in the treatment of rheumatoid arthritis is related to reducing the levels of inflammatory factors TNF-α， IL-1β and IFN-γ.

[Key words] Yishen Chubi Prescription; Rheumatoid arthritis; Inflammatory factors; Mechanism of function

类风湿关节炎（Rheumatoid Arthritis，RA）是一种较为常见的自身免疫性疾病，疾病发展后具有较高的致残性，并严重影响患者的生活和日常工作[1]。因RA致病因素及病理机制复杂多变，临床尚无特效治疗药物，目前也多采用联合用药治疗，以提高临床疗效[2-3]，但同时或多或少也存在药物副作用。因此，如何提高疗效，并降低RA致残率及药物副作用，改善患者生活质量，仍是当前临床治疗及研究的重点。中医药在RA的临床治疗有悠久的历史，且具有确切的疗效。……