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基于气相色谱-离子迁移谱技术鉴定内蒙古地区3 个品种羊肉挥发性代谢物

2021-12-23刘志红赵存谢遇春苏馨郭俊涛代东亮秦箐张崇妍兰茗熙蔡婷永泉王志新李金泉

肉类研究 2021年11期
关键词:气相色谱

刘志红 赵存 谢遇春 苏馨 郭俊涛 代东亮 秦箐 张崇妍 兰茗熙 蔡婷 永泉 王志新 李金泉

摘 要:分別选择放牧条件下6 只6 月龄体质量相近的乌珠穆沁羊、苏尼特羊和阿尔巴斯白绒山羊背最长肌为实验材料,采用气相色谱-离子迁移谱联用技术对3 个品种羊肉中的挥发性风味代谢物进行定性及定量测定,并对图谱差异进行分析,获得3 个品种羊肉的挥发性代谢物差异。运用主成分分析和偏最小二乘判别分析筛选乌珠穆沁羊肉与苏尼特羊肉、阿尔巴斯白绒山羊肉的差异挥发性代谢物。结果表明:在3 种羊肉中共检测到52 种挥发性风味代谢物,其中筛选并定性出乌珠穆沁羊肉与苏尼特羊肉差异代谢物7 种,分别为乙酸乙酯、丙酮、乙醇、2-丁酮、丁醛、壬醛和苯甲醛;乌珠穆沁羊肉与阿尔巴斯白绒山羊肉差异代谢物7 种,分别为丙酮、乙酸乙酯、2-丁酮、乙醇、苯甲醛、2-戊酮和3-羟基-2-丁酮;苏尼特羊肉与阿尔巴斯白绒山羊肉差异代谢物10 种,分别为丙酮、乙醇、2-丁酮、壬醛、乙酸乙酯、异戊醛、3-羟基-2-丁酮、2-戊酮、苯甲醛和辛醛。

关键词:气相色谱-离子迁移谱;乌珠穆沁羊;苏尼特羊;阿尔巴斯白绒山羊;挥发性代谢物

Identification of Volatile Metabolites of Lame Meat from Three Ovine and Caprine Breeds in Inner Mongolia by

Gas Chromatography-Ion Mobility Spectrometry

LIU Zhihong1, ZHAO Cun1, XIE Yuchun1, SU Xin1, GUO Juntao1, DAI Dongliang1, QIN Qing1,

ZHANG Chongyan1, LAN Mingxi1, CAI Ting2, YONG Quan3, WANG Zhixin1, LI Jinquan1

(1.Engineering Research Center for Goat Genetics and Breeding, Inner Mongolia Autonomous Region, Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Autonomous Region, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China; 2.Agricultural and Animal Husbandry Technology Extension Center of Inner Mongolia Autonomous Region, Hohhot 010010, China; 3.Animal Disease Prevention and Control Center of East Ujimqin Banner, Xilingol 026000, China )

Abstract: In this study, six grazing Ujimqin sheep, six grazing Sunit sheep and six grazing Albas white cashmere goats with similar body mass were selected and slaughtered at six months of age to collect Longissimus dorsi muscles. The volatile flavor metabolites in the meat samples were determined qualitatively and quantitatively by gas chromatography-ion mobility spectrometry (GC-IMS), and differential volatile metabolites between the three breeds were identified by principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA). The results showed that 52 volatile flavor metabolites were detected in all samples, among which seven differential metabolites between Ujimqin and Sunit sheep meat were identified, namely ethyl acetate, acetone, ethanol, 2-butanone, butyraldehyde, nonanal and benzaldehyde; seven differential metabolites between Ujimqin sheep and Albas white cashmere goat meat, namely acetone, ethyl acetate, 2-butanone, ethanol, benzaldehyde, 2-pentanone and 3-hydroxy-2-butanone; and ten differential metabolites between Sunit sheep and Albas white cashmere goat meat, namely acetone, ethanol, 2-butanone, nonanal, ethyl acetate, isovaleraldehyde, 3-hydroxy-2-butanone, 2-pentanone, benzaldehyde and octanal.

Keywords: gas chromatography-ion mobility spectrometry; Ujimqin sheep; Sunite sheep; Arbas white cashmere goats; volatile metabolites

DOI:10.7506/rlyj1001-8123-20210524-149

中图分类号:S826.8                                         文献标志码:A 文章编号:1001-8123(2021)11-0024-07

引文格式:

刘志红, 赵存, 谢遇春, 等. 基于气相色谱-离子迁移谱技术鉴定内蒙古地区3 个品种羊肉挥发性代谢物[J]. 肉类研究, 2021, 35(11): 24-30. DOI:10.7506/rlyj1001-8123-20210524-149.    http://www.rlyj.net.cn

LIU Zhihong, ZHAO Cun, XIE Yuchun, et al. Identification of volatile metabolites of lame meat from three ovine and caprine breeds in Inner Mongolia by gas chromatography-ion mobility spectrometry[J]. Meat Research, 2021, 35(11): 24-30. DOI:10.7506/rlyj1001-8123-20210524-149.    http://www.rlyj.net.cn

羊肉富含人体所需的氨基酸、脂肪酸和矿物质等营养物质,并且味美、鲜嫩多汁,具有健脾胃、补血等重要作用,是深受大众喜爱的肉类之一[1]。……

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