吴芳 李莉 黄彭 邸阳阳 嫣珍 魏琴

摘要 [目的]研究艳山姜叶片和果实所含的化学成分，并测定叶片和果实中总黄酮的含量。[方法]采用系统预试验法对艳山姜叶片和果实的水、95%乙醇、石油醚提取液进行化学成分研究;以芦丁为对照品，采用NaNO2-Al（NO3）3-NaOH比色法测定艳山姜叶片和果实中总黄酮的含量。[结果]艳山姜叶片和果实中均含有酚类、鞣质、有机酸、甾体、三萜以及挥发油、总黄酮;叶片中可能含生物碱，果实中不含生物碱;叶片和果实中不含皂苷，叶片和果实中总黄酮含量分别为13.05、11.98 mg/g。[结论]该研究初步确定了艳山姜叶片和果实中化学成分类型并测定了总黄酮含量，可为进一步研究其活性成分的提取分离及探索其化学成分与药理活性之间的关系奠定基础。

关键词 艳山姜;叶片和果实;化学成分;预试验;总黄酮含量

中图分类号 R284 文献标识码 A

文章编号 0517-6611（2021）09-0172-03

doi：10.3969/j.issn.0517-6611.2021.09.046

Abstract [Objective]To study the chemical components of leaves and fruits of Alpinia zerumbet，and to determine the content of total flavonoids in leaves and fruits.[Method]The extraction of leaves and fruits with water，95% ethanol and petrol ether was respectively experimented by comprehensive preliminary tests.Using rutin as substance control，the contents of total flavonoids in leaves and fruits were measured by colorimetric method with sodium nitrite-aluminum nitrate-sodium hydroxide.[Result]The leaves and fruits of Alpinia zerumbet contained phenols，tannins，organic acids，steroids，triterpenoids，volatile oils and total flavonoids.There may be alkaloids in leaves but no in fruits.Saponins were not found in leaves and fruits.The contents of total flavonoids in leaves and fruits were 13.05 and 11.98 mg/g，respectively.[Conclusion]This study initially determines the types of chemical components in the leaves and fruits of Alpinia zerumbet，and determines the content of total flavonoids，which can lay a foundation for further research on the extraction and separation of its active components and the exploration of the relationship between its chemical components and pharmacological activities.

Key words Alpinia zerumbet;Leveas and fruits;Chemical components;Preliminary tests;Content of total flavonoids

艷山姜[Alpinia zerumbet（pers.）]，是姜科（Zingiberaceae）山姜素属（Alpinia Roxb.）植物，广布于我国南方各省区，是民间用于驱寒暖胃的良药[1];其果实具有温中燥湿、行气止痛、截疟的功效[2]，我国许多地区习用果实及根茎入药，而叶片常被废置丢弃。艳山姜的叶在巴西作为一种药茶在民间使用，具有降低血压、利尿和抗溃疡的作用[3]，国外也有对艳山姜叶挥发油应用研究的报道[4]。目前国内对于艳山姜挥发油成分研究较多[5-6]，对艳山姜化学组成的系统研究还比较缺乏。该研究对该植物果实和叶片中化学成分进行系统预试验，为其化学成分的系统研究和有效成分的分离奠定基础。……