周海珍 栗亚琼 王淑霞 王祎娟

摘 要：建立猪肉及猪肉制品中牛磺酸含量的高效液相色谱-串联质谱（high performance liquid chromatography-tandem mass spectrometry，HPLC-MS/MS）检测方法。样品经水超声提取后，经乙腈沉淀蛋白，正己烷除脂肪，采用HPLC-MS/MS法定量测定。结果表明：在1～1 000 ng/mL线性范围内，牛磺酸的回归方程呈良好的线性关系，R2>0.998，在猪肉和猪肉制品阳性样品中加标水平为20～50 mg/100 g时，牛磺酸的加标回收率为92.78%～104.96%，相对标准偏差为1.39%～3.80%;该方法的仪器检出限为0.3 ng/mL，仪器定量限为1 ng/mL，方法检出限为0.1 mg/100 g，方法定量限为0.5 mg/100 g;该方法检出限低，回收率、灵敏度、准确度和精密度均较高，且操作简便、快捷，适用于猪肉及猪肉制品中牛磺酸含量的准确定性定量。

关键词：猪肉;猪肉制品;高效液相色谱-串联质谱法;牛磺酸;检测

Determination of Taurine in Pork and Pork Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry

ZHOU Haizhen1， LI Yaqiong1，WANG Shuxia2， WANG Yijuan2

（1.Food Testing Department， Luohe Vocational College of Food， Luohe 462300， China;

2.Technical Center， Shineway Group Co. Ltd.， Luohe 462003， China）

Abstract： This study intended to develop a method for rapid determination of taurine in pork and pork products by high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）. The samples were ultrasonically extracted with water， added with acetonitrile to precipitate protein， and re-extracted with n-hexane to remove fat. Then the analytes were detected by HPLC-MS/MS. The matrix-matched calibration curve developed showed a good linearity within the concentration range from 1 to 1 000 ng/mL （R2 > 0.998）. The average recovery for positive samples spiked with taurine at concentration levels of 20–50 mg/100 g was from 92.78% to 104.96%， with relative standard deviation （RSD） between 1.39% and 3.80%. The instrumental detection limit and quantitation limit were 0.3 ng/mL and 1 ng/mL respectively; the method detection limit and quantitation limit were 0.1 mg/100 g and 0.5 mg/100 g respectively. The method is characterized by low detection limit， as well as high recovery， sensitivity， accuracy and precision. It is simple and quick to operate and suitable for the accurate determination of taurine in pork and pork products.

Keywords： pork; pork products; high performance liquid chromatography-tandem mass spectrometry; taurine; determination

DOI：10.7506/rlyj1001-8123-20210203-030

中圖分类号：TS252.7                                         文献标志码：A 文章编号：1001-8123（2021）02-0035-06

引文格式：

周海珍， 栗亚琼， 王淑霞， 等. 高效液相色谱-串联质谱法测定猪肉及猪肉制品中牛磺酸含量[J]. 肉类研究， 2021， 35（2）： 35-40. DOI：10.7506/rlyj1001-8123-20210203-030.    http：//www.rlyj.net.cn

ZHOU Haizhen， LI Yaqiong， WANG Shuxia， et al. Determination of taurine in pork and pork products by high performance liquid chromatography-tandem mass spectrometry[J]. Meat Research， 2021， 35（2）： 35-40. DOI：10.7506/rlyj1001-8123-20210203-030.    http：//www.rlyj.net.cn

牛磺酸，化学名为2-氨基乙磺酸，俗称牛胆碱，是一种存在于动物体内的含硫非蛋白氨基酸[1-2]。牛磺酸曾经被认为是含硫氨基酸的无功能代谢产物[3]，直到1975年Hayes等[4]报道，采用以酪蛋白为主要蛋白来源的饲料饲喂猫，因其缺乏牛磺酸，可引起猫的视网膜变性，若长期缺乏，可导致猫失明，从而引起了人们对牛磺酸营养作用的高度重视。

研究表明，牛磺酸对生长发育有一定影响，是人体条件性必需氨基酸[5]，具有促进脂肪和脂溶性物质吸收、提高机体免疫力、促进生长及保护细胞、改善心肌缺血损伤及促进蛋白质、氨基酸、糖代谢等生理学功能[6-8]，适量的牛磺酸能促进机体的激素分泌，增强和改善机体的各种功能，是调节机体正常活动的活性物质，而缺乏牛磺酸会导致多种疾病的发生[9]。基于……