刘亚虓 张潘潘 颜偌楠 戚巍琼 邓翀 姜祎 张化为

【摘 要】 目的：研究芦根对干酵母致发热小鼠的炎症因子含量的变化，探讨芦根解热的药理机制。方法：将60只小鼠随机分为空白组、模型组、阳性组和水样低、中、高剂量组共6个组，连续灌胃给药7 d，由于酵母混悬液致热，最后检测小鼠体温变化，并测定血清中白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、环磷酸腺苷（cAMP）和前列腺素E2（PGE2）含量的变化。结果：与模型组比较，阳性组和给药组小鼠体温下降较为显著（P<0.05）;给药组血清中各细胞因子及下丘脑中PGE2的含量显著降低（P<0.05）。结论：芦根对干酵母致热小鼠具有显著的解热效果，其作用机制可能与降低发热小鼠血清中IL-1β、TNF-α、cAMP含量以及抑制下丘脑组织中PGE2的释放有关。

【关键词】 芦根;解热;作用机制

【中图分类号】R285.5   【文献标志码】 A    【文章编号】1007-8517（2021）22-0025-04

Study on the Mechanism of Heat-clearing Effect of Reed Rhizome

LIU Yaxiao ZHANG Pan YAN Ruonan QI Weiqiong DENG Chong JIANG Yi ZHANG Huawei*

School of Pharmacy， Shaanxi University of Traditional Chinese Medicine， Xianyang 712046， China

Abstract：Objective To study the effect of Reed root on the content of inflammatory factors in mice with fever caused by dry yeast and to explore the antipyretic mechanism of Reed Root.Methods Sixty mice were randomly divided into six groups： blank group， model group， positive group and water sample low-dose， medium-dose and high-dose groups. The mice were given intragastric administration for 7 days continuously. The changes of body temperature and serum levels of serum interleukin-1β（IL-1β）， tumor necrosis factor-α（TNF-α）， cyclic adenosine monophosphate（cAMP） and prostaglandin E2（PGE2）were measured because of the fever induced by yeast suspension. Results Compared with the model group， the temperature of mice in the positive group and the Administration Group decreased significantly （P<0.05）， and the contents of cytokines in serum and PGE2 in hypothalamus decreased significantly （P<0.05）.Conclusions  Reed root of dry yeast to heat has significant antipyretic effect， its mechanism may be related to reduce fever in mice serum IL-1β， TNF-α， cAMP the content and inhibit the release of PGE2 in the hypothalamus organization concerned.

Keywords：Rhizoma Phragmitis; Antipyretic; Mechanism of Action

蘆根来源于禾本科植物芦苇Phragmites communis Trin.新鲜或干燥的根茎[1]，性味甘、寒，归肺、胃经，具有清热生津、除烦、止呕、利尿等作用，常被广泛用于治疗萎缩性胃炎、大叶性肺炎、便秘等疾病[2]，还有用其治疗干燥综合征的报道[3]。近年来，芦根在预防炎症、感冒等方面起到了良好的作用[4]。

发热是指机体的体温调定点在免疫系统和神经系统协调作用下上移而诱发的一种适应性反应，其作用机制是由致热原的刺激使体温中枢调定点上移[5]。发热通常是通过一些炎症因子含量的变化来判断，而发热又使机体内炎症因子和前列腺素产生和释放增多，引起全身炎症从而导致体温升高[6]。常用的致热原有：脂多糖、2，4-二硝基酚、干酵母等[7]。……