基于病理学评价淫羊藿女贞子对激素干预哮喘大鼠的影响

年宏蕾,刘仁慧*,许利平,杨燕,王秀娟*

(首都医科大学中医药学院，中医络病研究北京市重点实验室，北京 100069)

摘要：目的基于病理学评价研究药对淫羊藿女贞子对激素干预哮喘大鼠的影响。方法卵蛋白致大鼠慢性哮喘模型后，腹腔注射地塞米松模拟激素干预过程，在激素应用同时及撤停时合用中药淫羊藿女贞子，观察对肺组织病理、血及肺泡灌洗液(BALF)涂片嗜酸性粒细胞(EOS)数量的影响。结果激素干预组、淫羊藿女贞子合激素Ⅰ、Ⅱ组显著改善肺组织支气管黏膜上皮脱落、管腔阻塞程度(分别为0.391±0.200，0.429±0.200，0.429±0.200，P均<0.05)，降低BALF中EOS数量(分别为8.30±2.06，9.70±2.45，10.60±2.32，P<0.05或0.01)及肺组织总管壁厚度(分别为106.26±14.00，95.73±14.78，87.47±14.44，P均<0.01)；激素干预组、淫羊藿女贞子合激素干预Ⅰ组显著抑制血EOS数量(分别为5.30±2.31，5.50±1.51，P均<0.01)，改善肺组织EOS浸润(分别为0.420±0.200，0.348±0.200，P均<0.05)；淫羊藿女贞子合激素Ⅰ组可显著降低肺组织平滑肌厚度(16.46±4.17，P<0.05)。结论激素应用同时配伍淫羊藿女贞子可增强协同抗炎作用及抑制哮喘气道重塑的作用。

关键词：糖皮质激素；淫羊藿；女贞子；气道炎症；气道重塑；大鼠

DOI:10.13463/j.cnki.jlzyy.2015.08.022

中图分类号：R285.5文献标志码： A

文章编号：1003-5699(2015)08-0826-04

基金项目:国家自然科学基金资助项目(81373814)；北京中医药薪火传承“3+3”工程二室一站建设项目(2012-SZ-C-42)；北京市属高等学校高层次人才引进与培养计划项目(SIT&TCD201504097)。

作者简介:年宏蕾(1991-)，女，硕士研究生，主要从事中药及复方的药效及作用机制研究。

收稿日期:(责任编辑：赵玉芝2014-09-04)

*通信作者:刘仁慧，电话-(010)83911639，电子信箱-liurenhui995@163.com

王秀娟，电话-(010)83911624，电子信箱-wxj0517@sina.com

HerbaEpimediiandFructusLigustrilucidion pathology evaluationin

asthmatic rats with hormone intervention

NIAN Honglei,LIU Renhui*,XU Liping,YANG Yan,WANG Xiujuan*

(School of Traditional Chinese Medicine,Capital Medical University;

Beijing Key Laboratory of Traditional Chinese Medicine Collateral Disease Theory Research,Beijing 100069,China)

Abstract:ObjectiveTo investigate the effects of the combination of Herba Epimedii,Fructus Ligustrilucidi and glucocorticoids(GC) on the pathology change in the asthmatic rats.MethodsRat asthmatic model was established by sensitization and challenge with ovalbumin(OVA).Dexamethasone was injected into asthmatic rats with the oral gavage of Epimedium and Ligustrumlucidum at different treatment stage of GC withdraw.The pathological changes of lung tissue and the eosinophils (EOS) number in the branchoalveolar lavage fluids(BALF) and blood were observed.ResultsThe GC group and the first and second groups of the combination of Herba Epimedii,Fructus Ligustrilucidi and GC alleviated bronchial mucosa epithelial shedding and lumenobstruction (0.391±0.200,0.429±0.200,0.4290±0.200,P<0.05),reduced the EOS number in BALF (8.30±2.06,9.70±2.45,10.60±2.32,P<0.05 or 0.01),and decreased the total bronchial wall area in the lung tissue (106.26±14.00,95.73±14.78,87.47±14.44,P<0.01).The GC group and the first group of the combination of Herba Epimedii,Fructus Ligustrilucidi and GC reducedthe EOS number inthe blood (5.30±2.31,5.50±1.51,P<0.01) and the EOS infiltration in the lung tissue (0.42±0.200,0.348±0.200,P<0.05).The first group of the combination of Herba Epimedii,Fructus Ligustrilucidi and GC can reduce the smooth musche area in the lung tissue (16.46±4.17,P<0.05).ConclusionsThe combination of Herba Epimedii,Fructus Ligustrilucidi and GC hasbetter anti-inflammatory effects on the asthmatic model of rats,and play a protective role in inhibiting airway remodeling.

Keywords:asthma;glucocorticoids;Herba Epimedii;FructusLigustri Lucidi;airway inflammation;airway remodeling;rat

哮喘是全球范围内严重威胁公众健康的一种常见慢性病[1]，目前针对哮喘发病机制中气道炎症学说，糖皮质激素(glucocorticoids,GC)被认为是防治哮喘的首选药物，但能否有效抑制气道重塑尚存在争议[1-2]，且实践中使用GC也存在诸多不良反应[3]。现代学者认为，外源性超生理剂量的GC类同于“壮火”，发越肾气、肾精，致火旺阴伤，日久累及肾阳，最终形成阴阳两亏[4-5]，防治GC的不良反应当以补肾中阴阳为基本立法。……