针刺对抑郁症睡眠障碍大鼠海马组织及血清中SOD·MDA的影响

俞裕天1,袁青2,陈津岩3,谢津津1,郎建英4,麦嘉玲1,曾晓林1，汪满霞1，薛尔平1

(1.广州中医药大学，广州 510405；2.广州中医药大学针灸康复临床医学院，广州 510405；

3.广州中医药大学核医学教研室，广州 510405；4.广州中医药大学基础医学院，广州 510405)

摘要：目的观察针刺对抑郁症睡眠障碍(SDD)大鼠海马组织及血清中SOD活性和MDA含量的影响。方法正常组8只SD大鼠常规合笼饲养。32只SD大鼠接受为期21 d的孤养+CUMS法+REM睡眠剥夺法造模，其中28只完成造模,二次分组为模型组、针刺组、药物组、针药组,每组各7只。模型组仅造模不干预;针刺组取印堂、神庭、太冲(双)进行针刺;药物组给予舍曲林和阿普唑仑水溶液灌胃；针药组同时结合针刺组与药物组的两种干预方式;3组均连续干预7 d。结果SDD造模致大鼠海马组织〔(41.19±5.59)u/mL〕和血清〔(34.57±5.53)u/mL〕的SOD活性较正常组〔(60.19±8.85)、(56.20±13.68)u/mL〕明显下调(P<0.05),且致海马组织〔(5.12±1.22)nmol/mg〕和血清〔(6.22±0.96)nmol/mg〕的MDA含量较正常组〔(3.49±1.02)、(4.35±1.55)nmol/mg〕明显上调(P<0.05);针刺组海马组织〔(52.29±11.37)u/mL〕和血清〔(54.32±6.95)u/mL〕SOD活性均较模型组有明显的上调(P<0.05)，且仅针刺组的海马组织〔(3.86±0.80)nmol/mg〕MDA含量较模型组得到了明显的下调(P<0.05)；药物组仅上调了血清SOD活性〔(44.70±5.31)u/mL，P<0.05)〕；针药组上调了海马组织〔(51.95±7.58)u/mL〕和血清〔(48.23±6.70)u/mL〕的SOD活性(P<0.05)。结论SDD造模能明显加速衰老；3种干预手段均有一定的抗衰老作用，其中针刺组最优，针药组次之。

关键词：抑郁症;睡眠障碍；衰老；针刺；靳三针;大鼠

DOI:10.13463/j.cnki.jlzyy.2015.10.027

中图分类号：R246文献标志码： A

文章编号：1003-5699(2015)10-1060-04

基金项目:国家自然科学基金经费资助(81072878、81303041)。

作者简介:俞裕天(1987-)，男,博士，在站博士后，主要从事中医药及针灸防治脑病、神志病的临床与实验研究。

收稿日期:(责任编辑：赵玉芝2014-12-03)

*通信作者:袁青，电话-13902211008，电子信箱-yuanqing1005@126.com

Acupuncture on hippocampus and serum SOD activity and MDA content of SDD rats

YU Yutian1,YUAN Qing2,CHEN Jinyan3,XIE Jinjin1,LANG Jianying4,MAI Jialing1,ZENG Xiaolin1,WANG Manxia1,XUE Erping1

(1.Guangzhou University of Chinese Medicine,Guangzhou 510405,China;

2.Clinical Medical College of Acupuncture,Moxibustion and Rehabilitation,Guangzhou University of Chinese Medicine,

Guangzhou 510405,China;

3.Dept.of Nuclear Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510405,China;

4.College of Basic Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

Abstract:ObjectiveObservation on acupuncture on hippocampus and serum SOD Activity and MDA content of SDD Rats.MethodsThe 8 normal Sprague Dawley rats were caged together normally.The 32 Sprague Dawley rats were underwent a 21 d solitary raised+CUMS+REM sleep deprivation method modeling.The 28 rats successfully completed the modeling,and divided into model group,acupuncture group,medicine group,acupuncture with medicine group,each group with 7 rats.Model group rats,only modeling without intervention.Acupuncture group rats were acupuncture on acupoint Yintang,Shenting and Taichong (both) each day for 20 min with a 15 s stimulation every 10 min.Medicine group rats were gavaged administration with Zoloft and Alprazolam solution.Acupuncture with medicine group combined acupuncture and medicine intervention.Three intervention groups were given intervention for 7 days.ResultsSOD activity of hippocampal tissue (41.19±5.59) u/mL and serum (34.57±5.53) /mL of DD rats was significantly lower than the normal rats (P<0.05),and hippocampus tissue and serum MDA content significantly increased than the normal (60.19±8.85,56.20±13.68) u/mL rats (P<0.05);Acupuncture group’s SOD activity of hippocampal tissue (5.12±1.22) nmol/mg and serum (6.22±0.96) nmol/mg were up regulated than the model group (3.49±1.02),(4.35±1.55) nmol/mg (P<0.05),and only acupuncture group’s MDA content in hippocampus has been significantly down regulated (P<0.05);Medicine group’s SOD (52.29±11.37) u/ml activity of serum (54.32±6.95) u/ml were down regulated (P<0.05);Acupuncture combined with medicine group’s SOD activity (44.70±5.31)u/mL,P<0.05, of hippocampal tissue (51.95±7.58) u/ml and serum (48.23±6.70) u/ml were up regulated (P<0.05).ConclusionSleep deprivation depression modeling can significantly accelerate aging;3 ways of interventions on SDD rats have some anti-aging effects.And acupuncture group has the best effect with acupuncture combined with medicine group followed.

Keywords:depression;sleep deprivation;aging;acupuncture;Jin’s three technique;rat

抑郁障碍(Major Depressive Disorder,MDD)影响着全世界约16%的人口[1]。睡眠障碍是抑郁症状群的重要组成部分，是抑郁症最常见的症状之一，以入睡困难、睡眠不深及早醒为特征。睡眠障碍可加重抑郁症的病情，延缓抑郁症的康复进程。抑郁症睡眠障碍(sleep deprivation depression,SDD)的研究已成为人们关注的焦点[2]，对SDD的动物模型[2-3]也在逐渐增多。……