外湿对大鼠各肠段菌群的影响

王菁1,刘晓燕2*，黄晓宇2

(1.北京市宣武中医医院,北京 100050;2.北京中医药大学基础医学院，北京 100029)

摘要：目的通过人工控制湿度，观察大鼠空肠、回肠和结肠的大肠杆菌、乳酸杆菌和双歧杆菌的菌落数，进而阐述外湿对肠道的影响，为湿邪致病阐明机制。方法将SD雄性大鼠随机分为常湿组、高湿组、低湿组，饲养21 d后，取空肠、回肠和结肠，分别进行大肠杆菌、乳酸杆菌和双歧杆菌平板计数。结果高湿组大鼠的空肠〔(4.50±0.09) logN/mL〕和结肠中大肠杆菌菌落值〔(7.30±0.38) logN/mL〕明显升高(均为P<0.05);低湿组大鼠空肠乳酸杆菌明显增多〔(6.09±0.13) logN/mL〕,P<0.01；低湿组空肠、回肠和结肠的双歧杆菌均非常明显的增多〔(5.86±0.31),(5.94±0.33) ,(7.07±0.16) logN/mL〕,均为P<0.01；高湿组结肠双歧杆菌的菌落值较为明显的升高〔(6.60±0.22) logN/mL〕,P<0.05。结论外湿对机体肠道内环境有明显的影响作用，改变了肠道菌群结构，从而导致疾病的产生。

关键词：外湿；中医脾脏；肠道菌群；大鼠

DOI:10.13463/j.cnki.jlzyy.2015.10.023

中图分类号：R223.1文献标志码： A

文章编号：1003-5699(2015)10-1046-03

基金项目:国家科技支撑计划课题(2012BAJ02B05)；国家自然科学基金资助项目(81001482)。

作者简介:王菁(1987-)，女，硕士，医师，主要从事脾胃病相关机制的研究。

收稿日期:(责任编辑：赵玉芝2015-01-26)

*通信作者:刘晓燕，电话-13693318075，电子信箱-liuxy1088@sina.com

Effects of exopathic dampness on intestinal flora of rat

WANG Jing1,LIU Xiaoyan2*,HUANG Xiaoyu2

(1.Beijing Xuanwu TCM Hospital,Beijing 100050,China;

2.Preclinical Medical College of Beijing University of Chinese Medicine,Beijing 100029,China)

Abstract:ObjectiveTo obverse the number of E.coil,lactobacillus,bifidobacterium in jejunum,ileum and colon by controlling the relative humidity,and then to reveal the effect of exopathic dampness towards intestinal tract.MethodsThe mice were divided into normal temperature normal humidity environment (NNE),normal temperature high humidity environment (NHE),and normal temperature low humidity environment (NLE) group,6 mice in each group.After 21 days feed,5 cm samples were taken out from jejunum,ileum and colon respectively,and then put the three samples in 4ml normal saline.Finally test the number of E.coil,LBA and BFB in the three samples by using plate count method.ResultsThe numbers of jejunum 〔(4.50±0.09) logN/mL〕 and colon E.coil 〔(7.30±0.38) logN/mL〕 in NHE group were obviously higher than those of NNE group (P<0.05).The number of jejunum lactobacillus in NLE group was much increased,compared with that of NNE group 〔(6.09±0.13) logN/mL〕,P<0.01.The numbers of jejunum 〔(5.86±0.31) logN/mL〕,ileum 〔(5.94±0.33) logN/mL〕 and 〔(7.07±0.16) logN/mL〕 were obviously higher in NLE group than those of NNE group (P<0.01).The number of colon bifidobacterium in NLE group was higher,contrasted with that of NNE group 〔(6.60±0.22) logN/mL〕,P<0.05.ConclusionExopathic dampness impacted obviously on intestinal environment,change the structure of intestinal flora to cause disease.

Keywords:exopathic dampness;spleen of TCM;intestinal flora;mouse

湿邪有内外之分。外湿作为外感病的致病因素，属六淫之一，多为外伤雾露、冒雨涉水、坐卧湿地、居处潮湿、汗出贴衣、气候潮湿等所感受的外界湿邪。湿邪是引起人体疾病的常见重要因素和发病条件之一。《素问·阴阳应象大论》有云“风胜则动，热胜则肿，寒胜则浮，燥胜则干，湿胜则濡泻”。外湿侵入机体易伤脾。脾胃为水谷之海，可化生精微、津液，对津液的输布至关重要[1]，故湿邪外感，留滞体内，常先困脾，而使脾阳不振，运化无权，水湿停聚，发为腹泻[2]。而现代研究证明[3-5]，中医脾与肠道正常菌群之间存在着十分密切的关系，脾虚泄泻与肠道菌群的失调密切相关。……