补骨脂素对胃癌细胞株BGC-803增殖和凋亡的影响及其机制

闫伟伟1,3,章永红1,刘军楼1*,吴坚2,邹玺2,刘沈林2,杨继兵1,于希忠1

(1.南京中医药大学,南京 210023;2.南京中医药大学第一附属医院,南京210029;

3.南阳市第一人民医院,南阳 473010)

摘要：目的研究补骨脂素对人胃癌BGC-803细胞的增殖和凋亡的影响并探讨其机制。方法以不同浓度的补骨脂素作用于体外培养的胃癌细胞BGC-803，不同时间后，采用MTT法检测药物对肿瘤细胞生长抑制率，流式细胞仪检测细胞凋亡率,激光共聚焦显微镜观察F-actin形态学变化。结果补骨脂素对人胃癌细胞株BGC-803具有明显的生长抑制作用，并与药物剂量及处理时间成正相关。补骨脂素作用于细胞24、48、72 h后的IC50分别为92.6、25.54、7.68 μg/mL。流式细胞仪检测结果显示给药组早期凋亡的百分率显著高于对照组，细胞骨架蛋白F-actin呈现解聚状态。结论：补骨脂素可抑制胃癌BGC-803细胞增殖，诱导肿瘤细胞凋亡，其机制可能是药物引起了细胞骨架蛋白F-actin解聚。

关键词：补骨脂素；胃癌；抑制增殖；细胞凋亡；细胞骨架蛋白

DOI:10.13463/j.cnki.jlzyy.2015.10.028

中图分类号：R285.5文献标志码： A

文章编号：1003-5699(2015)10-1064-04

基金项目:江苏高校优势学科建设工程(中西医结合)资助项目(PAPD)；江苏省中医药局课题资助项目(JD11038)。

作者简介:闫伟伟(1988-)，男，2012级硕士研究生，主要从事中医肿瘤病学研究。

收稿日期:(责任编辑：赵玉芝2015-03-30)

*通信作者:刘军楼，电话-13585148565，电子信箱-liujunlou1975@126.com

Psoralen on proliferation and apoptosis of gastric cancer cell line BGC-803 and its mechanism

YAN Weiwei1,3,ZHANG Yonghong1,LIU Junlou1,WU Jian2,ZOU Xi2,LIU Shenlin2,YANG Jibing1,YU Xizhong1

(1.Nanjing University of Chinese Medicine,Nanjing 210023,China;

2.The First Hospital Affiliated to Nanjing University of Chinese Medicine,Nanjing 210029,China;

3.The First People's Hospital of Nanyang,Nanyang 473010,Henan Province,China)

Abstract:ObjectiveThis experiment aims at observing the inhibition effects of Psoralen and apoptosis on human gastric carcinoma BGC-803 cells,discusses the possible mechanisms.MethodsBGC-803 cells in culture medium were treated with different concentrations of Psoralen.After different time period,the inhibitory rate of the cells was measured by MTT assay.Cell apoptotic rate was detected by flow cytometry (FCM).The morphological changes of F-actin were observed by laser confocal microscopy.ResultsThe growth of human gastric carcinoma BGC-803 cells was significantly inhibited by Psoralen,as the concentration increasing and the work time extending,the inhibition grows,which could be concluded as time-dose dependence.IC50 of Psoralen was respectively 92.6,25.54,7.68 μg/mL after 24 hours,48 hours,72 hours.The early apoptotic rate of BGC-803 cells was significantly higher than the control group’s.F-actin cytoskeletal protein was showed from polymerization to depolymerization after Psoralen treatment.ConclusionPsoralen can obviously inhibit the proliferation and induce apoptosis of BGC-803 cells.The mechanisms may involve the depolymerization of F-actin after treatment with Psoralen.

Keywords:psoralen;gastric carcinoma;antiproliferation;apoptosis;cytoskeletal protein

胃癌是严重威胁人类健康的重大疾病之一，发病率及死亡率逐年增加，对社会及家庭带来了沉重的压力及负担[1-5]。中医药治疗恶性肿瘤毒副作用低且疗效显著，对防治胃癌有较大的潜力与优势。……