高血压肝火亢盛证动物模型生物学基础研究

刘杨

(山东中医药大学附属医院心内科，济南 250014)

摘要：目的研究高血压肝火亢盛证动物模型的生物学基础。方法Wistar和自发性高血压大鼠(spontaneously hypertensive rats，SHR)各20只，分别设为Wistar组和SHR组,观察2组大鼠的宏观表征、行为学及血压变化，用免疫组化法及Elisa测定大鼠脑内及血清中的NE含量。根据大鼠临床证候诊断标准对动物表征的证候属性进行判断。结果2组大鼠收缩压均会随着周龄增加而增加，SHR大鼠在各周龄明显高于Wistar大鼠，差异有统计学意义(P<0.05)；SHR大鼠随着周龄增加其易激怒的评分会逐渐增加，旋转耐受时间会随着周龄的增加而减少，而Wistar大鼠评分波动幅度及旋转耐受时间波动幅度不明显，2组大鼠不同时间易激怒评分差异和旋转耐受时间差异有统计学意义(P<0.05)；随着周龄的增加，2组大鼠5-HT在逐渐减小，NE在逐渐增加，SHR变化幅度明显大于Wistar，2组差异有统计学意义(P<0.05)。结论SRH大鼠在14～18周龄为肝火亢盛证的稳定期，和大鼠血液和脑内NE的含量有很大关系，可作为SHR肝火亢盛证的客观指标。

关键词：肝火亢盛证；自发性高血压大鼠；生物学基础

DOI:10.13463/j.cnki.jlzyy.2015.10.024

中图分类号：R259文献标志码： A

文章编号：1003-5699(2015)10-1049-04

基金项目:山东省科技发展计划项目(2014GSF119011);山东省中医药科技发展计划(2013ZDZK-018);山东省中医药科技发展计划(2011-083)。

作者简介:刘杨(1981-)，男，硕士，主治医师，主要从事中医治疗心血管疾病疗效评价的研究。

收稿日期:(责任编辑:赵玉芝2015-07-07)

Biological base of anger hyperactivity hypertension animal model

LIU Yang

(Department of Cardiology,The Affiliated Hospital of Shandong University of Traditional Chinese Medicine,

Ji’nan 250014,China)

Abstract:ObjectiveTo study the biological basis of hyperactivity hypertension anger animal model.MethodsDynamic acquisition of Wistar and spontaneously hypertensive rats (spontaneously hypertensive rats,SHR),each with 20,were set Wistar group and SHR group,observed macroscopic characterization,behavior and changes in blood pressure of two groups rats,determinate NE content in brain and rat serum by immunohistochemistry Elisa.Judgment of animal attributes characterizing based on clinical symptoms in rats standards.ResultsThe two groups SBP increases with weeks,SHR rats was significantly higher than in the week-old Wistar rats,the difference was statistically significant (P<0.05);SHR rats with weeks increase irritability ratings will gradually increase,the rotation time will increase tolerance and reduce weeks of age,and Wistar rats rates fluctuations and volatility is not obvious rotation endurance time,two groups of rats at different times irritability rating differences and rotation endurance time was significantly (P<0.05);With the increase of weeks old,5-HT in the two groups were gradually reduced,NE gradually increased,SHR change was greater than that Wistar,groups the difference was statistically significant (P<0.05).ConclusionSRH rats 14-18 weeks of anger hyperactivity stabilization period,and the content of the NE of blood and brain in rats have a great relationship,as an objective indicator of SHR anger hyperactivity.

Keywords:anger hyperactivity;spontaneously hypertensive rats;biological basis

原发性高血压会引起机体心脑血管等形成器质性的损害，导致脑卒中、心肾衰竭、冠心病等的出现，在临床上有高致残率和高致死率的特点。根据人类疾病来建立动物模型可有效的阐述疾病发病机理，并对人类疾病进行有效的预防和诊断，对治疗方法的研究有很大的应用价值，结合病症和动物模型可为中医基础和临床提供良好的桥梁作用，其稳定情况是进行中医研究重要的前提条件[1-2]。自发性高血压大鼠(SHR)动物模型和人类的原发性高血压有被国际公认的极相似的发病机制。有研究结果显示[3]，SHR体征、症状、器官及组织学病理和生化指标等都会随着大鼠周龄的增加而发生相应的变化，然而目前关于自发性高血压的中医证候时间属性的研究还有待完善。……