王雨婷 陈曼 王秀景 邓天琦 奚嘉 蒋小飞

*基金項目：江苏省徐州市科学技术局项目（KC22186）

第一作者简介：王雨婷（1997-），女，硕士研究生，研究方向：妇科肿瘤。

△通信作者：蒋小飞，E-mail：1004357062@qq.com

摘要：目的  本研究基于网络药理学探讨痛经丸治疗子宫腺肌病痛经的有效成分、关键靶标及作用机制，旨在为临床应用提供理论依据、为进一步的研究提供思路和研究方向。方法  使用TCMSP和UniProt数据库获取痛经丸的有效成分及对应靶点基因，利用GeneCards、DisGeNET等疾数据库获取AM和痛经的相关疾病靶点基因。利用Venny2.1平台得到药物-疾病的交集靶点基因，通过Cytoscape 3.9.1软件中构建“药物-有效成分-靶蛋白-疾病”可视化网络，再利用String数据库构建蛋白互作（PPI）网络；最后基于Metascape数据库进行GO及KEGG分析。结果  共筛选出痛经丸药物有效成分共141个，获得药物-疾病交集靶点基因21个；GO 功能富集分析分别获得了484项生物过程（BP）、10项细胞成分（CC）和30项分子功能（MF），涉及的信号通路主要有PI3K-Akt信号通路、p53信号通路、IL-17 信号通路、TNF 信号通路、NF-κB 信号通路等和HIF-1信号通路。结论  痛经丸可能是通过多成分-多靶标-多通路协同作用于细胞增殖、侵袭和转移、血管生成、细胞凋亡、炎症反应、间充质细胞转化等病理过程，从而发挥治疗子宫腺肌病痛经的作用。

关键词：痛经丸；子宫腺肌病；痛经；网络药理学；拓扑分析；作用机制

中图分类号：R271.11+3    文献标志码：A    文章编号：1007-2349（2024）05-0022-09

Exploration the Action Mechanism of Menstrual Pain Pills in the Treatment of Dysmenorrhea in Adenomyopathy Based on Network Pharmacology

WANG Yu-ting1， 2， CHEN Man1， 2， WANG Xiu-jing1， DENG Tian-qi2， XI Jia2， JIANG Xiao-fei2

（1. Anhui University of Traditional Chinese Medicine， Hefei 230038， China;2. Xuzhou Hospital of Traditional Chinese Medicine， Xuzhou 221000， China）

【Abstract】Objective：To explore the active ingredients， key targets and action mechanism of Menstrual Pain Pills in the treatment of dysmenorrhea in adenomyopathy based on network pharmacology in order to provide theoretical basis for clinical application and ideas and research direction for further research. Methods： The active ingredients and corresponding target genes of Menstrual Pain Pills were obtained by TCMSP and UniProt databases， and the target genes of AM and dysmenorrhea related diseases were obtained by GeneCards and DisGeNET disease databases. The intersection target genes of herb-disease were obtained using Venny2.1 platform. The visualization network of “herb-ingredient-targetprotein-disease” was constructed via Cytoscape 3.9.1 software， and the protein interaction （PPI） network was constructed by using String database. Finally， GO and KEGG analysis were made on the base of Metascape database. Results： A total of 141 active ingredients of Menstrual Pain Pills were screened， and 21 genes of herb-disease intersection were obtained. And 484 biological processes （BP）， 10 cell components （CC） and 30 molecular functions （MF） were obtained respectively by GO functional enrichment analysis. The signaling pathways that were involved mainly included PI3K-Akt signaling pathway， p53 signaling pathway， IL-17 signaling pathway， TNF signaling pathway， NF-κB signaling pathway and HIF-1 signaling pathway. Conclusion： Menstrual Pain Pills may play a role in the treatment of dysmenorrhea in adenomyopathy through the synergistic action of multi-ingredient， multi-target and multi-pathway on cell proliferation， invasion and metastasis， angiogenesis， apoptosis， inflammatory response， mesenchymal cell transformation and other pathological processes.

【Key words】Menstrual Pain Pills; Adenomyopathy; Dysmenorrhea; Network Pharmacology; Topological Analysis; Action Mechanis

子宫腺肌病（adenomyosis，AM）以子宫内膜腺体和间质向肌层的浸润性生长为病理表现，以盆腔疼痛（表现为痛经、性交痛和慢性盆腔痛）、异常子宫出血和生殖功能受损为主要临床症状［1］。……