周玲 李秋月 雷从改 符芳霞 李云 程斌 覃锐 刘天密

摘要 选择经常检出的磺胺嘧啶作为研究对象，通过测定不同保存条件下罗非鱼中不同浓度磺胺嘧啶的稳定性，确定罗非鱼磺胺类药物监测中的样品保存条件和运输温度条件。结果表明，样品中不同浓度的磺胺嘧啶均有不同程度的降解，但因保存条件的不同，降解速率各不相同。在常温条件下保存，罗非鱼样品中磺胺嘧啶的浓度越高，降解速度越慢;在冷藏条件下保存，罗非鱼样品中磺胺嘧啶浓度越高，也呈降解速度越慢的趋势;在冷冻条件下保存，罗非鱼样品中磺胺嘧啶的降解速度没有明显的变化。罗非鱼样品中不同浓度的磺胺嘧啶在3种保存条件下（常温25～28 ℃，冷藏0～4 ℃，冷冻-18～-22 ℃）的降解速率为常温>冷藏>冷冻。因此，用于磺胺嘧啶检测的罗非鱼样品应贮存于冷冻条件下，并尽快分析。

关键词 罗非鱼样品;磺胺嘧啶;降解规律

中图分类号 S 948  文献标识码 A  文章编号 0517-6611（2021）16-0174-04

doi：10.3969/j.issn.0517-6611.2021.16.047   开放科学（资源服务）标识码（OSID）：

Degradation of Sulfadiazine under Different Storage Conditions in Tilapia Fish Muscle Samples

ZHOU Ling，LI Qiu-yue，LEI Cong-gai et al

（Testing Center for Aquatic Products Quality and Safety of Hainan Province，Haikou， Hainan 570206）

Abstract The frequently detected sulfadiazine was selected as the research object. To ensure the transportation temperature and storage conditions of tilapia fish muscle samples for monitoring of sulfonamides by detecting the stability of different concentrations of sulfadiazine in tilapia fish muscle samsulfonamidesples under different storage conditions. The results showed that sulfadiazine of different concentrations in tilapia fish muscle samples degraded with various degrees， however， the degradation rate varied due to different storage conditions. The degradation rate of sulfadiazine in tilapia fish muscle samples were both slower with the increase of sulfadiazine concentration at room temperature and cold storage. But there was no significant change in the degradation rate of sulfadiazine in tilapia fish muscle samples stored under freezing condition. The result also showed that the degradation rate of sulfadiazine at different concentrations in tilapia fish muscle samples under three storage conditions were in order of room temperature condition > refrigerated condition > freezing condition. Therefore， tilapia fish muscle samples for sulfadiazine detection should be stored under freezing conditions and analyzed as soon as possible.

Key words Tilapia fish muscle samples;Sulfadiazine;Degradation law

磺胺類药物为人工合成的抗菌药，它具有抗菌谱较广、性质稳定、使用简便等优点，在水产养殖中广泛使用[1]。但是，如果磺胺类药物使用不当或使用时未遵守其休药期，可造成药物在动物组织中的残留[2-3]。残留的药物不仅会对人的健康造成潜在危害，引起过敏反应、肾脏损坏、白细胞减少症等，还是水产品出口贸易中的主要障碍[4]。2015年美国FDA发布拒绝进口产品通报，其中我国广西、海南等地多批罗非鱼因兽残问题被拒绝入境。因为磺胺……