刘合霞 刁慧玲 舒杨 谭肖玲 韦晓晓 李妍 龙开芸 苏雅萍 赵娇妹 李博

摘要 磷脂酰乙醇胺結合蛋白 （phosphatidyl ethanolamine binding protein， PEBP）在花的形态建成中具有重要作用，为了解该基因家族在金花茶开花过程中的调控作用，通过对金花茶的花瓣转录组测序数据进行生物信息学分析，从转录组数据库中筛选金花茶（Camellia nitidissima）PEBP基因，并对其蛋白质性质、功能结构域、进化关系及开花过程中的表达模式进行了分析。结果表明，金花茶花瓣转录组中共含有9个PEBP基因家族成员，可以分为FT-like、TFL1-like、MFT-like、PEBP-like 4个亚基因家族;该蛋白家族主要由酸性、亲水的稳定蛋白构成，定位于细胞核中;该基因家族Motif具有一定的保守型，且所包含的保守基序数目及种类存在一定差异。在金花茶开花过程中，CnPEBP 4基因的表达量不断增高，该基因可能对金花茶花瓣形态建成具有一定的调控作用;而其他基因家族成员在金花茶花瓣组织中表达量较低或不表达。鉴定获得了金花茶PEBP基因家族成员的序列和部分功能信息，为研究金花茶开花调控提供了理论参考，为金花茶开花调控机理的研究奠定基础。

关键词 PEBP基因家族;金花茶;保守基序;进化分析;表达量分析

中图分类号 S-685.14  文献标识码 A

文章编号 0517-6611（2021）13-0103-05

doi：10.3969/j.issn.0517-6611.2021.13.025

开放科学（资源服务）标识码（OSID）：

Transcriptome wide Identification and Expression Profiling of PEBP Gene Family in Camellia nitidissima

LIU He xia， DIAO Hui ling， SHU Yang et al

（College of Biology and Pharmacy， Yulin Normal University， Yulin， Guangxi 537000）

Abstract Phosphatidyl ethanolamine binding protein （PEBP） plays an important role in the formation of flower.In order to konw the function of PEBP gene family of Camellia nitidissima in blossom， PEBP gene family was selected from the transcriptome database of petal in Camellia nitidissima with bioinformatics analysis， and their protein properties， functional domains， evolutionary relationships and expression patterns during flowering were analyzed.The results showed that there were a total of 9 PEBP gene families in the petals transcription of Camellia nitidissima， which could be divided into four sub gene families， such as FT like， TFL1 like， MFT like， PEBP like， etc.The protein family is mainly composed of acidic， hydrophilic and stable proteins located in the nucleus.In addition， the Motif of this gene family had a certain conservative type， and there were some differences in the number and types of conservative motifs.During the flowering process of Camellia nitidissima， the expression level of CnPEBP4 gene increases continuously， which may had a certain regulatory effect on the petal morphological formation of Camellia nitidissima， but other gene family members showed low or no expression.In this study， the sequence and partial functional information of the PEBP gene family members of Camellia nitidissima were identified， which provided theoretical reference for the study of flowering regulation and laid a foundation for the study of flowering regulation mechanism of Camellia nitidissima.

Key words PEBP gene family;Camellia nitidissima;Conserved motif;Phylogenetic analysis;Expression analysis

在植物分类系统中，金花茶（Camellia nitidissima）属于山茶科（Theaceae）山茶属（Camellia）植物，于1965年在我国广西首次被发现，主要分布于我国广西南部地区及越南北部地区，是山茶属中唯一具有金黄色花瓣的珍稀植物类群，素有“茶族皇后”“植物界的大熊猫”的美誉[1]。……