许刚 何纯青 张飞 克里木 尹东峰 吾木尔 刘剑 贾勇

[摘要]目的：采用低溫3D打印技术制备复合万古霉素的PLGA/TCP多孔骨缺损修复支架材料，并对其生物相容性和抑菌效果进行检测，旨在使支架材料促进骨缺损修复的同时，兼具局部抗感染的能力。方法：按15%PLGA+1.5%TCP+1.5%万古霉素的比例，应用低温快速3D打印技术制备了40%、60%和80%三种不同孔隙率的骨缺损修复支架材料，并对该材料进行表面形态观察和测量、细胞毒性检测、万古霉素缓释能力实验和抑菌效果检测。结果：扫描电子显微镜观察支架材料见其互连通性好，大孔直径在550μm左右，孔连通率在90%以上。三种不同孔隙率的支架材料均无细胞毒性，孔隙率为80%的支架材料在万古霉素缓释能力和抑菌效应方面要显著优于孔隙率为40%和60%的支架材料。结论：应用低温3D打印技术可以直接在生物墨水中加入注射用万古霉素粉剂，制备出具有良好三维结构的复万古霉素/PLGA/TCP的多孔支架材料，该支架孔通率高，互连通性好，而且当孔隙率为80%的支架材料在万古霉素缓释能力及抑菌效应方面有着更优的表现，可视为一种具有潜在临床应用价值的支架材料，尤其适用于感染性骨缺损的修复。

[关键词]低温3D打印;骨缺损修复支架;聚乳酸-羟基乙酸;磷酸三钙;万古霉素

[中图分类号]R622    [文献标志码]A    [文章编号]1008-6455（2021）05-0059-04

Preparation and Properties Study on the Low Temperature 3D Printing Composite Vancomycin/PLGA/TCP Scaffold

XU Gang1，HE Chun-qing1，ZHANG Fei1，KE Li-mu1，YIN Dong-feng2，WU Mu-er1，LIU Jian1，JIA Yong1

[1.Department of Traumatic Orthopedics，Orthopedic Center of PLA（Joint group）;2.Department of Pharmacy，General Hospital of Xinjiang Military Region，Urumuqi 830000，Xinjiang，China]

Abstract： Objective  To design via low temperature 3D printing composite scaffold （PLGA/TCP） made of poly （lactic-co-glycolic acid） （PLGA） and tricalcium phosphate （TCP） as a basic carrier， incorporating vancomycin powder. The structural and performance of such composite scaffold is tested， so as to make the scaffolds promote bone defect repair and have anti-infection ability. Methods  According to the ratio of 15%PLGA+1.5%TCP+1.5% vancomycin， such scaffolds were fabricated using a low-temperature rapid-prototyping 3D printing process，and the porosity for 40%， 60% and 80% were designed. The surface morphology of the scaffolds were observed and measured， biocompatibility was evaluated by the cytotoxicity test of the bone marrow mesenchymal cells. The vancomycin release and antibacterial test were also observed in our study. Results  The large pores diameter of the vancomycin incorporated PLGA/TCP scaffold is about 550μm and the pore connectivity is 90%. All scaffolds have good biocompatibility and no cytotoxicity. The results of vancomycin release and antibacterial test for scaffold with 80% porosity were significantly superior to those with porosity of 40% and 60%. Conclusion  A new type of composite vancomycin incorporated PLGA/TCP porous scaffold was fabricated using low temperature rapid prototyping technology， and the scaffold with 80% porosity has better performance in Vancomycin release and antibacterial test， which means that scaffold can be a scaffold material with potential clinical application value， especially suitable for the repair of infectious bone defect.

Key words： low temperature 3D print; bone defect repair scaffold; poly（lactic-co-glycolic acid）（PLGA）; tricalcium phosphate （TCP）; vancomycin

临床中由于骨与关节感染、结核或肿瘤侵蚀、严重创伤等导致的骨缺损比较常见，而大段骨缺损的修复成为骨科领域最为棘手的难题之一[1-3]。……