王楠 许良 吴国明

[摘要]目的：研究川芎嗪对体外培养大鼠成纤维细胞增殖及碱性成纤维细胞因子（Basic fibroblast growth factor，bFGF）、转化生长因子β1（Transforming growth factor β1，TGF-β1）表达的影响，从而探讨川芎嗪抑制瘢痕形成的作用机制。方法：体外分离培养大鼠皮肤成纤维细胞，采取MTT法及荧光染色检测不同浓度的川芎嗪对大鼠成纤维细胞增殖能力的影响，ELISA法测定大鼠皮肤瘢痕成纤维细胞TGF-β1和bFGF的表达量。结果：在三种川芎嗪浓度条件下（1mg/ml，10mg/ml，50mg/ml），大鼠皮肤成纤维细胞增殖能力有不同程度的抑制作用，抑制作用与川芎嗪浓度成正比，在50mg/ml浓度时，抑制作用最为明显。采用ELISA法测定培养上清TGF-β1和bFGF的分泌量，发现实验组两者含量均明显低于对照组，其中浓度为50mg/ml时，含量最低（P<0.05）。结论：川芎嗪能够明显抑制体外培养的大鼠皮肤成纤维细胞的增殖能力，降低TGF-β1和bFGF的表达量，从而在一定程度上抑制大鼠皮肤成纤维细胞的活性。

[关键词]川芎嗪;皮肤;成纤维细胞;瘢痕;转化生长因子β1;碱性成纤维细胞因子

[中图分类号]R619+.6    [文献标志码]A    [文章编号]1008-6455（2021）05-0056-03

Effect of Ligustrazine on Fibroblast Proliferation and the Expression of TGF-β1 and bFGF in Fibroblasts

WANG Nan，XU Liang，WU Guo-ming

（Jiangnan Hospital Affiliated to Zhejiang Traditional Chinese Medical University，Hangzhou 311201，Zhejiang，China）

Abstract： Objective  To investigate the influence of ligustrazine on the proliferation and expresson of TGF-β1 and bFGF for human normal and hypertrophic scar dermal fibroblasts cultured in vitro and to explore the relevant mechanism. Methods  The rat skin fibroblasts were isolated and cultured in vitro， and MMT assay was used to detect the effects of ligustrazine at various concentrations on the rat skin scar fibroblasts in vitro. ELISA was carried out to determine the contents of TGFβ1 and bFGF in rat skin scar fibroblasts. Results  Under 3 ligustrazine concentrations （1mg/ml， 10mg/ml， 50mg/ml）， the rat skin fibroblasts were suppressed to various degrees， and such suppression was positively correlated with concentration. Typically， the most obvious suppression was attained at the concentration of 50mg/ml. Besides， TGF-β1 and bFGF contents were measured by ELISA， the results suggested that， the expression contents in experiment group were lower than those in control group， and the lowest contents were detected at the concentration of 50mg/ml （P<0.05）. Conclusion  Ligustrazine markedly suppresses the activity of rat skin fibroblasts cultured in vitro， and reduces the TGF-β1 and bFGF contents. Typically， the efficacy peaks under a relatively suitable concentration， thus partially suppressing the activity of rat fibroblasts.

Key words： ligustrazine; skin; fibroblasts; scar; transforming growth factor-β1（TGF-β1）; basic fibroblast growth factor（bFGF）

外傷所致皮肤损伤（尤其深度烧伤）愈合后形成增生性瘢痕（Hypertrophic scar，HS）的现象极为常见[1]。瘢痕不但影响美观，严重时还可导致局部畸形或功能障碍，从而给患者后期治疗造成沉重的经济和心理负担[2]。病理性瘢痕作为皮肤损伤最常见的并发症之一，一直是外科修复领域亟待解决的难题;抑制皮肤增生性瘢痕的生长及纤维化病变的进展，是近年研究的焦点[3]。尽管临床治疗瘢痕的方法众多（如手术治疗、放射治疗、药物治疗等），但疗效都并不尽如人意[4]。川芎嗪是临床上广泛使用的一种心脑血管药物，具有改善血液流变学和清除氧自由基的作用[5]。……