刘立兵 员丽娟 陈敏娜 王金凤 付琦 孙晓霞 王建昌

摘  要：为实现肉制品中鸭源性成分的定量检测，基于微滴式数字聚合酶链式反应技术（droplet digital polymerase chain reaction，ddPCR），以鸭生长激素（growth hormone，GH）基因为靶基因，建立肉制品中鸭源性成分的定量检测方法。结果表明：所建立的ddPCR方法能特异性检测鸭源性成分，灵敏性为11.1 copies/μL;根据鸭肉质量（mg）与DNA质量浓度（ng/μL）、DNA质量浓度（ng/μL）与鸭GH基因拷贝数浓度（copies/μL）之间的线性关系，建立鸭肉质量（x）与鸭GH基因拷贝数浓度（y）之间的关系式为 ;模拟混合样本中鸭肉粉质量分数5%～100%时，鸭源性成分检测的绝对误差均小于±1.52%，变异系数均小于10.38%，回收率为98.35%～125.32%;在88 份商品化肉制品中，11 份肉制品中检出鸭源性成分，检出率为12.5%，且鸭源性成分含量为18.6%～87.4%。本研究所建立的鸭源性成分ddPCR方法有较好的准确性和较强的适用性，能够应用于肉制品中鸭源性成分的定量检测。

关键词：肉制品;鸭源性成分;生长激素基因;微滴式数字聚合酶链式反应;定量检测

Quantitative Detection of Duck-Derived Ingredients in Meat Products by Droplet Digital Polymerase Chain Reaction

LIU Libing1， YUAN Lijuan2， CHEN Minna1， WANG Jinfeng1， FU Qi1， SUN Xiaoxia1， WANG Jianchang1，*

（1.Technology Center of Shijiazhuang Customs， Shijiazhuang   050051; 2.Technology Center of Xian Customs， Xian   710075）

Abstract： A droplet digital polymerase chain reaction assay （ddPCR） based on the duck growth hormone gene （GH） as the target gene was developed for the quantitative detection of duck-derived ingredients in meat products. The results showed that the assay was highly specific for the detection of duck derived ingredients， but not the other tested animal components， and its limit of detection was 11.1 copies/μL. According to the linear relationship between the mass of duck meat （mg） and DNA concentration （ng/μL）， and that between DNA concentration （ng/μL） and the number of GH gene copies （copies/μL）， the linear equation between the mass of duck meat （X） and the number of GH gene copies （Y） was established as follows： . When the percentage of duck meat powder ranged from 5% to 99% in simulated mixed samples， the absolute error was less than ± 1.52%， and the coefficient of variation was less than 8.30% with a recovery between 98.35% and 108.78%. Duck -derived ingredients were detected at levels of 18.6%–87.4% in 11 out of 88 samples of commercial meat products， with a detection rate of 12.5%. The developed ddPCR assay demonstrated good accuracy and applicability for the quantitative determination of duck-derived component in commercial meat products.

Keywords： meat products; duck-derived ingredients; growth hormone gene; droplet digital polymerase chain reaction; quantitative determination

DOI：10.7506/rlyj1001-8123-20210119-013

中圖分类号：TS207.3              文献标志码：A               文章编号：

引文格式：

刘立兵， 员丽娟， 陈敏娜， 等. 基于微滴式数字聚合酶链式反应技术的肉制品中鸭源性成分的定量检测[J]. 肉类研究， 2021， 35（3）：  . DOI：10.7506/rlyj1001-8123-20210119-013.    http：//www.rlyj.net.cn

LIU Libing， YUAN Lijuan， CHEN Minna， et al. Quantitative detection of duck-derived ingredients in meat products by droplet digital polymerase chain reaction[J]. Meat Research， 2021， 35（3）：  . DOI：10.7506/rlyj1001-8123-20210119-013.    http：//www.rlyj.net.cn

肉制品是我國居民食品消费的重要组成部分，主要以猪肉、鸡肉、牛肉、羊肉和鸭肉为主。一些不法商贩为获得高额利润，使用低价优质肉冒充高价优质肉，如根据鸭肉的纹理色泽和牛羊肉更为接近，采用牛羊肉精、肉粉或香精等对鸭肉进行浸泡，以此掩盖其掺假行为，用鸭肉冒充牛羊肉进行销售[1-3]。……