26份水稻材料白叶枯病和稻飞虱病抗性基因检测与分析
2021-03-01宗凯盛旋吕侠影程潇李云飞孙娟娟李克娟余晓峰姚剑
宗凯 盛旋 吕侠影 程潇 李云飞 孙娟娟 李克娟 余晓峰 姚剑



摘要 对26份水稻材料进行抗病基因检测,包括8个白叶枯病抗性基因(Xa1、 xa5、 Xa7、xa13、 Xa21、 Xa23、 Xa26、 Xa27)和2个稻飞虱病抗性基因(Bph14、 Bph15)。其中23份水稻材料中含有Xa1基因材料20份,含xa5基因材料20份,含Xa7基因材料23份,含xa13基因材料12份,含 Xa21基因材料9份,含 Xa23基因材料23份,其中抗性4份,感性19份,含Xa26基因材料23份,含Xa27基因材料21份,其中抗性5份,感性16份。另有3份材料进行稻飞虱病抗性基因Bph14、Bph15检测,检测结果表明这3份材料均含有Bph14、Bph15基因。选取13个已报道的基因标记或连锁标记检测26份杂交稻材料,初步确定了其抗白叶枯病和抗稻飞虱病等位基因分布和利用情况,为水稻基础材料的筛选、亲本材料的选择及育种应用提供了良好的参考。
关键词 水稻品系;抗性基因;白叶枯病;鉴定
中图分类号 S435.11 文献标识码 A 文章编号 0517-6611(2021)01-0088-04
doi:10.3969/j.issn.0517-6611.2021.01.023
Abstract Twenty-six rice varieties were tested for disease resistance genes, including eight bacterial blight resistance genes (Xa1, xa5, Xa7, xa13, Xa21, Xa23, Xa26, Xa27) and two rice planthopper disease resistance genes (Bph14, Bph15). Among them, 23 rice materials contained 20 Xa1 genetic materials, 20 xa5 genetic materials, 23 Xa7 genetic materials, 12 xa13 genetic materials, 9 Xa21 genetic materials, 23 Xa23 genetic materials, including 4 resistance, 19 susceptibility, 23 Xa26 genetic materials and 21 Xa27 genetic materials, including 5 resistance and 16 susceptibility. Three other materials were tested for Bph14 and Bph15 genes of rice planthopper disease resistance. The results showed that all the three materials contained Bph14 and Bph15 genes. The distribution and utilization of alleles for resistance to bacterial blight and rice planthopper disease in 26 hybrid rice materials were preliminarily determined by screening 13 reported gene markers or linkage markers, which provided a good reference for screening basic rice materials, selection of parent materials and breeding application.
Key words Rice strains;Resistance gene;Bacterial blight;Identification
水稻是我國重要的主粮之一,而白叶枯病和稻飞虱病是水稻生产中重要的,也是常见的病虫害[1-2],针对这2种病虫害的抗性基因定位和克隆一直备受关注。目前已有超过26个白叶枯病抗性基因和20个褐稻飞虱病抗性基因被定位,其中7个白叶枯病抗性基因和1个褐稻飞虱病抗性基因已经被克隆,与此同时,与这些抗性基因紧密连锁或者共分离的分子标记被开发出来,利用这些已鉴定的分子标记可以快速准确地筛选和鉴定出水稻材料中所含的抗性基因类型,辅助水稻育种亲本材料的筛选,显著加速育种过程。该研究筛选8个白叶枯病抗性基因……
