张俊林 樊靖 胡玉春 杨浩 胡绍庆 沈柏春

摘要：为建立‘哈伯’南天竹组织培养和种苗繁育技术体系，以半木质化带芽茎段为外植体材料开展植株再生研究。通过观察对比试验法、L9（34）正交试验设计完全随机法、极差分析、显著性检验、LSD多重比较，探讨了‘哈伯’南天竹组培的最适培养基配方。试验结果表明：最佳诱导培养基为MS + 6-BA2.0 mg/L + IBA0.1 mg/L +蔗糖30 g/L，诱导萌动率71.77%，成活率85.51%；最佳增殖培养基为WPM +6-BA1.5 mg/L+ IBA 0.01 mg/L +蔗糖30 g/L，增殖系数6.3；最佳生根培养基为1/2 MS+ IBA 0.5 mg/L + NAA 1.0 mg/L+蔗糖20 g/L +AC 0.2 g/L，生根率97.63%；试管苗移入泥炭土：珍珠岩=3：2（V/V）混合基质中，移栽成活率96.67%。该试验建立了高效稳定的组培快繁技术体系，得到的组培苗后代能够稳定的保持母本优良性状，为工厂化育苗提供了技术支撑。

关键词：‘哈伯’南天竹；组织培养；种苗繁育；再生；增殖

中图分类号：S688.4文献标志码：A论文编号：cjas20200300087

Nandina domestica‘Harbour Dwarf’： Tissue Culture and Rapid Propagation

Zhang Junlin1， Fan Jing1， Hu Yuchun1， Yang Hao1， Hu Shaoqing2， Shen Bochun1

（1Hangzhou Landscaping Incorporated， Hangzhou 31000， Zhejiang， China；

2Building Engineering College， Zhejiang Sci-Tech University， Hangzhou 310018， Zhejiang， China）

Abstract： The aim of this study is to establish a system of tissue culture and seedling production for Nandina domestica‘Harbour Dwarf’. The stem segments of semi-lignified band buds were used as explants to conduct regeneration study. The comparative experiments， completely randomized orthogonal experiment design of L9（34）， range analysis， significance test and LSD multiply comparative analysis were used to discuss the optimal culture medium. The results showed that the highest frequency of shoot induction （71.77%） and survival rate（85.51%） were achieved on MS + 6-BA 2.0 mg/L + IBA 0.1 mg/L + sucrose 30 g/L. The highest proliferation coefficient （6.3） was obtained on WPM +6-BA 1.5 mg/L + IBA 0.01 mg/L + sucrose 30 g/L. Microshoots exhibited best rooting response on 1/2 MS+ IBA 0.5 mg/L + NAA 1.0 mg/L + sucrose 20 g/L + AC 0.2 g/L by producing the highest frequency of root formation （97.63% ）. Rooted plantlets were transferred to artificial mixture medium （peat： perlite=3：2.V/V） under the green house condition with the survival rate up to 96.67%. An efficient propagation system of Nandina domestica‘Harbour Dwarf’was established， and the plantlets could steadily inherit the character of parents. The present system provides technical support for large scale plant production.

Keywords： Nandina domestica‘Harbour Dwarf’； Tissue Culture； Seedling Production； Regeneration； Proliferation

0引言

南天竹（Nandina domestica）为小檗科南天竹属常绿灌木[1]，是一种具有观赏、生态、药用等多种价值的树种[2]，其叶、果、株型皆具极高的观赏价值，广泛用于园林绿化中[3-4]。南天竹原产中国华北、华中、华东及华南部分地区，日本亦有分布[5]，经过自然演化及栽培驯化已形成多个栽培变种，常见的栽培变种有红果南天竹、玉果南天竹、五彩南天竹和藍果南天竹、狭叶南天竹、栗木南天竹和圆叶南天竹[6]，茎干笔直，高约2 m，少分枝或无分枝。‘哈伯’南天竹（Nandina domestica‘Harbour Dwarf’）原产地为美国北卡罗莱纳州[7]，生长缓慢，株高40～80 cm，枝叶浓密，株形紧凑，基部萌蘖性强；叶片对生，2～3回羽状复叶，偶有4回，小叶纺锤形，长4～8 cm，宽1～2 cm，先端钝尖，基部楔形，全缘，薄革质，属矮生类型南天竹新品种。……