高艾英 于洋

摘 要：为研究不同消解方法对蜂蜜中矿物质元素测定的影响，找到适用于蜂蜜的最佳前处理方法，本文分别采用湿法消解、干法消解和微波消解3种方式对蜂蜜进行预处理，于电感耦合等离子体质谱（ICP-MS）优化条件下，测定16种标准元素物质的加标回收率。结果表明，3种消解方法的测定结果均处于参考范围内，其中微波消解法消解最彻底，回收率基本处于99.17%～109.38%;湿法消解处理中，As、Pb、Zn和Ca元素回收率与微波消解方式相比无明显差异，且Mg的回收率高于微波消解，其余元素回收率处91.6%～103.8%;干法消解回收率效果最差，与其他两种消解方式回收差异明显。综合比较来看，微波消解是最适用于蜂蜜样品的预处理方式。

关键词：蜂蜜;微波消解;干法消解;湿法消解;ICP-MS

Abstract：In order to study the influence of different digestion methods on the determination of mineral elements in honey and find the best pretreatment method for honey， this study used wet digestion， dry digestion and microwave digestion to pretreat honey respectively. Under the optimized condition of ICP-MS， the recovery of 16 standard elements was determined. The results showed that the results of the three digestion methods were in the reference range， among which microwave digestion was the most thorough， and the recovery rate was basically 99.17%～109.38%; in the wet digestion， the recovery rates of as， Pb， Zn and Ca were no significant difference compared with microwave digestion， and the recovery rate of Mg was higher than that of microwave digestion， and the recovery rate of other elements was 91.6%～103.8%; the dry digestion was 91.6%～103.8% The recovery effect is the worst， and the recovery difference is obvious with the other two digestion methods. Microwave digestion is the most suitable pretreatment method for honey samples.

Key words：Honey; Microwave digestion; Dry digestion; Wet digestion; ICP-MS

我国蜜蜂养殖产业已有2 000多年的历史，蜂蜜产量巨大，且出口量可观。蜂蜜作为一种优质的天然营养品，营养价值全面，含有多种对人体有益的功能性物质，如人体所需的氨基酸、维生素B、维生素D、维生素E等以及钾、铁、钙、钠、镁等矿物质元素[1-3]，其保健功能与药用价值得到广泛认可。其中，矿物質作为人体的重要组成物质，具有维持机体正常运作的生理功能，不能在体内自行合成，必需通过外界饮食摄入，然而过多或过少的摄入量都会导致身体出现机能紊乱[4]，如钠元素摄入含量不足，会出现眩晕、低血压、脉搏加快等症状，而摄入过多则会导致消肿、高血压、肾病等中毒病症[5];同时过高或过低的钾元素含量都会导致人体心跳过快、心律不齐[6]，因而矿物质含量在一定程度上决定着人体机能的运作状况。……