袁晓庆 李莉 刘言娟 王振

摘要[目的]建立大孔吸附樹脂分离纯化牛蒡叶中绿原酸的工艺。[方法]通过单因素试验研究提取液种类、浓度、pH、提取温度、料液比以及提取时间等参数对绿原酸提取率的影响，确定最佳提取工艺;以大孔吸附树脂对牛蒡叶中绿原酸的分离效率为评价指标，通过静态和动态吸附/解吸附试验优化分离纯化工艺。[结果]pH=1的蒸馏水为提取溶剂，料液比1∶20（g∶mL）、提取温度80 ℃、回流1 h时对牛蒡叶中绿原酸的提取效果最佳，平均提取率为1.82%;考察了6种大孔吸附树脂对牛蒡叶绿原酸的分离纯化性能，以吸附/解吸附性能为评价指标，确定了LX-218为最佳大孔吸附树脂。LX-218型MAR分离纯化牛蒡叶绿原酸的最佳工艺条件为：上样量为30 BV（树脂床体积），上样浓度为0.7倍提取原液浓度（相当于原生药），上样液pH=3，以4 BV/h流速吸附，5 BV pH=5的60%乙醇以5 BV/h的流速解吸附。在优化的工艺条件下，牛蒡叶绿原酸得率为84.41%，纯度为55.26%。[结论]LX-218型大孔吸附树脂对牛蒡叶绿原酸有较好的吸附容量和解吸附率，优化的生产工艺条件适用于牛蒡叶绿原酸的工业化生产。

关键词牛蒡叶;大孔吸附树脂;绿原酸;分离纯化;工艺优化

中图分类号R284.2文献标识码A

文章编号0517-6611（2020）04-0168-07

doi：10.3969/j.issn.0517-6611.2020.04.049

开放科学（资源服务）标识码（OSID）：

Study on the Technology of Separation and Purification of Chlorogenic Acid from Burdock Leaves by Macroporous Adsorption Resin

YUAN Xiao-qing，LI Li，LIU Yan-juan et al（Linyi University，Linyi，Shandong 276000）

Abstract[Objective]To establish the optimum technological conditions for the separation and purification of chlorogenic acid from burdock leaves by macroporous adsorption resin（MAR）.[Method]The optimum extraction process was obtained by studying the effects of extractant type，concentration，pH，extraction temperature，ratio of solid-liquid ratio and extraction time with single factor experiment.Based on separation efficiency of chlorogenic acid from burdock leaves，the optimal separation and purification processes were obtained by static and dynamic adsorption/desorption experiments.[Result]The optimum extraction process as follows：the extractant was distilled water with pH=1，the ratio of solid-liquid ratio was 1：20（g：mL），the extraction temperature was 80 °C，the reflux time was 1 h.The average extraction rate was 1.82% with the above extraction process.The separation and purification performance of six kinds of macroporous adsorption resins for burdock chlorogenic acid were investigated.Taking adsorption / desorption performance as the evaluation index，LX-218 was determined as the best macroporous adsorption resin.The optimal process conditions on LX-218 MAR for the separation and purification of burdock chlorophyllic acid as follows：the loading amount was 30 BV（resin bed volume），the loading concentration was 0.7 times the concentration of the extracted stock solution（equivalent to raw material），and the loading solution pH=3，adsorption at 4 BV/h flow rate，respectively.For the desorption process，chlorogenic acid loaded MAR column was desorbed by 5 BV ethanol-aqueous solution（60∶40，V∶V） with pH value of 5 at flow rate of 5 BV/h.Under the optimal conditions，the yield of chlorogenic acid from burdock leaves and purity were 84.41% and 55.26%，respectively.[Conclusion]LX-218 macroporous adsorption resin has good adsorption and desorption performance for chlorogenic acid.The optimized technique is suitable for the industrial production of chlorogenic acid from burdock leaves.

Key wordsBurdock leaves;Macroporous adsorption resin;Chlorogenic acid;Separation and purification;Process optimization

牛蒡又名大力子、恶实、牛蒡子，属于菊科类植物，近年来作为保健型蔬菜受到人们的青睐。它全身是宝，其子、根均可入药，富含糖、纤维素、蛋白质、钙、铁等人体所需的多种营养物质和矿物质等[1-2]。我国是牛蒡生产大国，仅临沂市兰陵县的庄坞、层山、二庙等乡镇，种植面积达0.67万hm2以上，县外发展种植基地0.80万hm2以上，年总产量约70万t，占全国牛蒡产量的50%以上。……