李程 黎妍妍 杨小琼 李春黎 许玉享 王林 陈守文 杨勇

摘要  将实验室保藏的青枯病拮抗菌ZM9和YH-22配制成复合菌剂，并将万寿菊提取物和复合菌剂联合配制成协同菌剂，通过分析万寿菊提取物对青枯菌的抑制效果，复合菌剂和协同菌剂抑制青枯菌的效果。结果表明，万寿菊提取物对青枯菌具有抑制作用，且万寿菊提取物用量与抑制效果呈显著负相关（Pearson=-0.997，P=0.047）;万寿菊提取物对青枯病拮抗菌有一定的促进作用;复合菌剂和协同菌剂对青枯菌均有抑制作用，协同菌剂抑菌效果显著高于复合菌剂;综合分析表明万寿菊提取物可以与青枯病拮抗菌联合施用，并能增强对青枯菌病原菌抑制效果。研究结果为烟草青枯病生防菌剂的研制拓展了新的方向。

关键词  烟草青枯病;拮抗菌;万寿菊提取;复合菌剂;协同菌剂

中图分类号  S  435.72文献标识码  A

文章编号  0517-6611（2020）04-0128-04

doi：10.3969/j.issn.0517-6611.2020.04.038

开放科学（资源服务）标识码（OSID）：

The Inhibitory  Effect of  Synergistic and Compound Antagonistic Agent on Tobacco Bacterial Wilt

LI  Cheng1，LI  Yan-yan2，YANG Xiao-qiong1 et al  （1.School of  Life  Sciences，Hubei University，Wuhan，Hubei  430062;2.Tobacco Research Institute of Hubei Province，Wuhan，Hubei   430030）

Abstract  In this experiment，the antagonistic bacteria of tobacco bacterial wilt ZM9 and YH-22 preserved in our laboratory were prepared into compound bacterial agent.Marigold rhizome extract and above-mentioned compound bacterial agent were combined to prepare synergistic bacterial agent.By analyzing and comparing the inhibitory effect of marigold rhizome extract，compound bacterial agent and synergistic bacterial agent on tobacco bacterial wilt， it was found that marigold rhizome extract had an inhibitory effect on the tobacco bacteria wilt，and the amount of marigold rhizome extract was negatively correlated with the inhibitory effect （Pearson=-0.997，P=0.047）.Besides，marigold rhizome extract can promote the growth of antagonistic bacteria of tobacco bacterial wilt to some extent.According to the statistical analysis of bacteriostatic circle and plate colony number，both the synergistic bacterial agent and compound bacterial agent had inhibiting effect on tobacco bacterial wilt，but the inhibitory effect of synergistic bacterial agent was significantly higher than that of compound bacterial agent.Comprehensive analysis showed that marigold rhizome extract could combine with antagonistic bacteria of tobacco bacterial wilt ZM9 and YH-22 to enhance the inhibitory effect of tobacco bacterial wilt.The resuts of this study opened a new direction for the development of tobacco bacterial wilt biocontrol agents.

Key words  Tobacco bacterial wilt;Antagonistic bacteria;Marigold rhizome extract;Compound bacteria agent;Synergistic bacteria agent

煙草青枯病对烟叶生产的影响备受关注[1-2]，烟草青枯病防治成为烟叶生产相关领域研究热点之一[3-5]。目前防治青枯病的策略主要包括化学防治、生物防治和农艺防治[5]，其中化学防治存在后期防效差，长期施用导致病菌产生抗药性[6]，抗病品种选育成功率低是农业防治中关键性难题[7-8]，生物防治方法因其安全无公害和长效等优点成为烟草青枯病防治的热点[2，7]，其中采用生物菌剂防治烟草青枯病的方式得到相关研究领域认可[8]，且复合生防菌的应用成为主流趋势[9]。研究虽然取得了一些效果[10]，但青枯病蔓延趋势并未得到有效的控制[11]。……