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高效液相色谱法定性定量测定油梨果实中的可溶性糖成分

2017-08-09葛宇曹剑秋司雄元胡福初李章

热带农业科学 2017年7期
关键词:高效液相色谱

葛宇+曹剑秋++司雄元++胡福初+李章++臧小平++马蔚红

摘 要 分析油梨果实可溶性糖成分及含量,为以后深入分析、利用其成分提供一定理论基础。对所采集的3份油梨,应用高效液相色谱仪测定其可溶性糖组分及含量。结果显示,3份油梨果肉中的可溶性总糖含量为(13.19 ± 0.32)~(25.94 ± 0.98)(mg/g FW),其可溶性糖由甘露庚酮糖、甘露庚糖醇、果糖及葡萄糖等4个成分组成,以甘露庚酮糖为主;可溶性总糖、甘露庚酮糖、甘露庚糖醇、果糖及葡萄糖含量在3份油梨果肉中差异均显著。3份油梨种子中的可溶性总糖含量为(20.47±0.52)~(29.41±0.60)(mg/g FW),其可溶性糖同样由甘露庚酮糖、甘露庚糖醇、果糖及葡萄糖等4个成分组成,以甘露庚糖醇为主;可溶性总糖、甘露庚酮糖、甘露庚糖醇、果糖及葡萄糖含量在3份油梨种子中差异均显著。3份油梨果肉和种子之间可溶性总糖和各可溶性糖成分平均含量差距大小依次為:甘露庚糖醇>果糖>甘露庚酮糖>葡萄糖>可溶性总糖。

关键词 油梨 ;果肉和种子 ;可溶性糖成分 ;高效液相色谱

中图分类号 S667.9 文献标识码 A Doi:10.12008/j.issn.1009-2196.2017.07.018

Identification and Quantitation of Soluble Sugar Components of

Avocado Fruit by High Performance Liquid Chromatography

GE Yu1) CAO Jianqiu2) SI Xiongyuan3)

HU Fuchu4) LI Zhang2) ZANG Xiaoping1) MA Weihong1)

(1 Haikou Experimental Station, CATAS, Haikou, Hainan 570102;

2 College of Horticulture and Landscape Architecture, Hainan University, Haikou, Hainan 570228;

3 Biotechnology Center, Anhui Agricultural University, Hefei, Anhui 230036;

4 Institute of Tropical Fruit Tree, Hainan Academy of Agricultural Sciences /

Hainan Key Laboratory of Tropical Fruit Tree Biology, Haikou, Hainan 571100)

Abstract Avocado fruits (Persea americana Mill.) were collected from 3 accessions of avocado germplasm and their soluble sugar components and contents were determined. The results showed that the contents of total soluble sugar in the pulps of the avocado fruits of the 3 avocado accessions ranged from (13.19 ± 0.32) to (25.94 ± 0.98) mg/g FW. The soluble sugar components were composed of mannoheptulose, perseitol, fructose and glucose, and dominated by mannoheptulose. There were significant differences in the contents of total soluble sugar, mannoheptulose, perseitol, fructose and glucose among the avocado fruits of 3 avocado accessions. The total soluble sugar contents in the seeds of the 3 avocado accessions were at the range of between (20.47 ± 0.52) and (29.41 ± 0.60)mg/g FW. The soluble sugar component also included perseitol, mannoheptulose, fructose and glucose, dominated by perseitol. There were great differences between the contents of total soluble sugar, mannoheptulose, perseitol, fructose and glucose of the seeds of 3 avacado germplasms. The average contents of total soluble sugar and other soluble sugar components in the fruit pulp and seeds of 3 avocado accessions was in the order of perseitol > fructose > mannoheptulose > glucose > total soluble sugar.

Keywords Persea americana Mill. ; pulp and seed ; soluble sugar components ; high performance liquid

油梨(Persea americana Mill.)为樟科(Lauraceae)鳄梨属(Persea)植物,中文又名鳄梨、牛油果。油梨起源于中美洲、墨西哥热带湿润地区及海拔较高的山地森林或热带高原,是著名的热带、亚热带果树,同时也是木本油料树种之一[1]。油梨主要有墨西哥、危地马拉和西印度群岛3个生态型,目前全世界有40多个国家栽培,其中墨西哥、危地马拉及美国南部栽培最多[1]。油梨于1918年被引种到中国台湾省,现在海南、广东、广西、贵州、云南、台湾等地均有栽培[2-5]。中国油梨……

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