魏冬梅+尹钢+刘锐+王蔷+王超男+邱学荣

[摘要]目的 针对性地收集齐齐哈尔脑卒中患者的生物样本，构建标准化、高质量的脑卒中生物样本库，为齐齐哈尔市脑卒中疾病数据分析和临床决策提供样本资源和数据支撑平台。方法 制订标准化操作流程，筛选入库对象，收集符合标准的患者血清、血浆、白细胞及从白细胞中提取的DNA和RNA，保存于-80℃冰箱或液氮中，进行DNA和RNA质量鉴定，确保提供高质量的临床样本资源。结果 从2013年起，该样本库已收集753例患者，收集血清、血浆样本各3008份，白细胞2253份，DNA、RNA样本各3770份。电泳结果显示，RNA电泳均出现18S和28S两条带，无弥散现象，且28S条带亮度约为18S条带亮度的2倍，DNA电泳条带清晰，完整性良好。结论 初步建立了标准化、高质量的齐齐哈尔地区脑卒中生物样本库，以进一步探索和利用脑卒中样本资源，为今后脑卒中患者个体化治疗提供帮助。

[关键词]脑卒中；生物样本库；齐齐哈尔

[中图分类号] R743.3 [文献标识码] A [文章编号] 1674-4721（2016）06（a）-0008-04

[Abstract]Objective To targeted collect the biological samples of patients with cerebral apoplexy in Qiqihar，to construct a standardized biological sample bank of cerebral apoplexy with high quality and provide sample sources and data supporting platform for the data analysis and clinical decisions for cerebral apoplexy in Qiqihar.Methods The standardized operative process was made，the object was selected，blood serum，blood plasma，white cells，DNA and RNA extracted from the white cells of the standardized patients were collected and stored in the refrigerator at -80℃ or liquid nitrogen.The quality of DNA and RNA was detected so that clinical sample sources with high quality were to be provided.Results From 2013，a total of 753 patients were enrolled in the sample bank.Samples of blood serum and blood plasma were 3008 respectively，white cells were 2253 and DNA and RNA were 3770 respectively.The results of electrophoresis showed the RNA electrophoresis had both 18S and 28S stripes without dispersion and the lumen of 28S stripe was as twice as that of the 18S stripe.DNA electrophoresis had clear stripes with good integrity.Conclusion The standardized biological sample banks of cerebral apoplexy with high quality in Qiqihar is primarily established to further explore and apply the data sources of cerebral apoplexy，which can provide help for individualized treatment of patients with cerebral apoplexy in the future.

[Key words]Cerebral apoplexy；Biological sample bank；Qiqihar

脑卒中是齐齐哈尔地区的高发疾病，严重危害人类健康，具有高发病率、高复发率、高致残率和高致死率的特点[1]。2008年原卫生部发布的《我国居民第三次死因回顾抽样调查报告》指出，脑血管疾病已成为导致我国居民死亡的首要原因，其中以脑卒中造成的损害为重[2]，给患者家庭和社会带来了沉重的负担。

近年来，临床研究人员逐渐意识到样本资源对于科研工作的重要性，开始重视临床生物样本的大量收集和规范化的管理工作[3-5]。……