刘志强　徐振东　沈富毅　张玥琪　李海冰

(同济大学附属第一妇婴保健院麻醉科，上海　200040)

·论著·

异氟烷宫内暴露对胎鼠海马神经元凋亡的影响

刘志强徐振东沈富毅张玥琪李海冰

(同济大学附属第一妇婴保健院麻醉科，上海200040)

摘要目的：观察异氟烷宫内暴露对胎鼠海马神经元凋亡的影响。方法： 将妊娠19 d的SD大鼠随机分为异氟烷麻醉组(I组)和对照组(C组)，I组孕鼠接受3 h的1.3%异氟烷吸入麻醉，C组吸入空气。采用原位末端标记法(TdT mediated dUTP nick end labeling，TUNEL)观察胎鼠大脑海马CA1区神经元在异氟烷暴露结束后0、3 h和9 h时间点的凋亡情况。结果： I组和C组海马CA1区神经元在异氟烷暴露后0、3 h和9 h均见凋亡；2组异氟烷暴露后0 h和3 h凋亡指数差异均有统计学意义(P<0.05)，暴露后9 h凋亡指数差异无统计学意义(P>0.05)。结论： 1.3%异氟烷宫内暴露不会加重胎鼠海马CA1区神经元的凋亡，在暴露后早期反而有减缓作用，提示胎儿期可能不是异氟烷发育神经毒性的易损期。

关键词异氟烷；宫内暴露；凋亡；海马神经元

中图分类号R329.2+5

文献标识码A

Effect of In-Utero Exposure to Isoflurane on Apoptosis of Hippocampal Neuron in Fetal RatsLIUZhiqiangXUZhendongSHENFuyiZHANGYueqiLIHaibing

DepartmentofAnesthesiology,ShanghaiFirstMaternityandInfantHospital,TongjiUniversity,Shanghai200040,China

AbstractObjective： To observe the effect of in-utero exposure to isoflurane on apoptosis of hippocampal neuron in fetal rats. Methods： SD rats at gestational day 19 were randomly assigned into the isoflurane anesthesia group(group I) and the control group(group C). The rats in group I were exposed to 1.3% isoflurane for 3 h,while group C were exposed to air. The Apoptosis status of neurons in hippocampal CA1 region of fetal brains were observed by using TdT mediated dUTP nick end labeling(TUNEL) 0 h,3 h, and 9 h after exposure to isoflurane. Results： There were a certain amount of apoptotic neurons in hippocampal CA1 region in both group I and group C 0 h,3 h,and 9 h after the exposure.There were significant differences regarding apoptosis index(AI) 0 h and 3 h after the exposure between the two groups (P<0.05). However, there was no significant difference regarding the AI between the two groups 9 h after the exposure (P>0.05). Conclusions： The in-utero exposure to 1.3% isoflurane does not increase the apoptosis of neurons in hippocampal CA1 region of fetal rats. Furthermore, it may alleviate the apoptosis during the early period.The result suggests that fetus period may not be the vulnerable period for isoflurane-induced neurotoxicity.

Key WordsIsoflurane；In-utero exposure；Apoptosis；Hippocampal neuron

研究[1]发现，麻醉药可导致幼年动物大脑神经元过量凋亡，干扰突触的发育和塑形，影响神经元细胞骨架的正常形成及神经胶质细胞的发育和成熟等，并可能造成持续至成年阶段的神经认知能力削弱。与出生后的幼年动物接受全身麻醉不同，胎儿大脑处于发育的更早期，在其未脱离母体环境的情况下受到全身麻醉药的暴露是否会引发上述改变值得探究。本研究通过使孕鼠吸入异氟烷造成胎鼠大脑的宫内暴露，观察胎鼠海马CA1区神经元不同时间点的凋亡状况，以明确以异氟烷为代表的全身麻醉药对胎儿期大脑组织形态的影响，为进一步了解其对中枢神经系统发育期学习记忆能力的影响及机制提供依据。……