过常发　孙笑天　王文硕　王春生

(1. 复旦大学附属中山医院心外科，上海　200032; 2. 复旦大学附属华山医院胸心外科，上海　200040)

·论著·

二烯丙基三硫化物对内皮细胞缺氧复氧损伤的保护作用

过常发1孙笑天2王文硕1王春生1

(1. 复旦大学附属中山医院心外科，上海200032; 2. 复旦大学附属华山医院胸心外科，上海200040)

摘要目的：探究以二烯丙基三硫化物为主要成分的大蒜素对心血管系统发挥保护作用的机制。方法： 以内皮细胞缺氧复氧模型为对象，采用细胞计数试剂盒(CCK8)评价内皮细胞活力的变化；用激光共聚焦显微镜测定内皮细胞线粒体膜电位的改变；将细胞接种于matrigel，观察血管形成能力的差异。结果： 二烯丙基三硫化物在质量浓度为0.2 μg/mL时对缺氧复氧损伤的保护作用最强，其主要机制是通过减少线粒体膜电位去极化水平使内皮细胞在经历缺氧复氧后仍维持正常的增殖分化能力。结论： 二烯丙基三硫化物通过线粒体途径减轻内皮细胞缺氧复氧的损伤。

关键词氧化应激；线粒体膜电位；增殖；分化

中图分类号R329.2

文献标识码

Protective Effect of Diallyl Trisulfide on Hypoxia-Reoxygenation Injury of Endothelial CellsGUOChangfa1SUNXiaotian2WANGWenshuo1WANGChunsheng1

1.DepartmentofCardiacSurgery,ZhongshanHospital,FudanUniversity,Shanghai200032,China; 2.DepartmentofCardiothoracicSurgery,HuashanHospital,FudanUniversity,Shanghai200040,China

AbstractObjective： To explore the mechanism of the protective effect of allicin, of which the main composition is diallyl trisulfide, on cardiovascular system. Methods： The endothelial cell model of hypoxia-reoxygenation injury was the research object. Vitality change of endothelial cells was evaluated by using CCK8. Confocal laser scanning microscope was used to detect the change of mitochondrial membrane potential in endothelial cells. Endothelial cells were planted onto matrigel so as to observe the difference regarding the ability of angiogenesis. Results： It showed that diallyl trisulfide performed the best protective effect at the concentration of 0.2 μg/mL, and its main mechanism based on maintaining the normal ability of proliferation and differentiation of endothelial cells after hypoxia-reoxygenation process by reducing the depolarization level of mitochondrial membrane potential. Conclusions： Diallyl trisulfide alleviates the hypoxia-reoxygenation injury of endothelial cells through the pathway involved with mitochondria.

Key WordsOxidative stress；Mitochondrial membrane potential；Proliferation；Differentiation

大蒜素及其组成成分在人体内能够发挥多种有益的生理作用，包括降脂、降压、抗血栓、增强免疫以及抗肿瘤作用[1-2]。多年来，人们探索了大蒜素制备产物在体内外对心血管疾病的防治作用[3]。随着硫化氢作为体内第三种重要气体信号分子被发现，大蒜素作为该气体分子的天然供体愈发受到重视[4]。在大蒜素的各种制备产物中，二烯丙基三硫化物(diallyl trisulfide，DATS)因其分子结构具有3个巯基而被认为抗氧化作用最强[5]。……