李建华 周洁 陆素青 欧阳新平 陈歆 张涛

[摘要] 目的 研究姜黄素对PC-3细胞增殖活性的影响。 方法 以不同浓度（10、20、40、60、80μmol/L）的姜黄素及顺铂作用于体外培养的PC-3细胞24 h，以中效浓度（40μmol/L）的姜黄素及顺铂作用于体外培养的PC-3细胞4、12、24、48、72 h，后用MTT法检测在不同浓度及时间作用下PC-3细胞的抑制率，应用流式细胞仪检测PC-3细胞周期的变化。 结果 姜黄素对PC-3细胞的增殖抑制作用与顺铂相似（P＞0.05），具有剂量时间依赖性，并随着药物浓度及时间的增加，抑制率逐渐增高，流式细胞仪检测显示：姜黄素和顺铂组都能使PC-3细胞生长明显阻滞于G2/M期，两组比较差异无统计学意义（P＞0.05）。各实验组与空白对照组比较均有显著性差异（P＜0.05）。 结论 姜黄素可以明显抑制PC-3细胞的增殖，在临床治疗前列腺癌方面有着良好的应用前景。

[关键词] 姜黄素；人前列腺癌细胞系；细胞增殖；细胞凋亡

[中图分类号] R965.2???[文献标识码] A???[文章编号] 2095-0616（2012）15-29-03

Inhibitory effect of curcumine on prostate cancer cell line PC-3

LI?Jianhua1??ZHOU?Jie2??LU?Suqing3??OUYANG?Xinping4??CHEN?Xin4??ZHANG?Tao5

1.Department of Pharmacy,the First People's Hospital of Chenzhou, Chenzhou 423000,China;2.General Surgery,the 163rd Hospital of PLA,Hengyang 421001,China;3.Department of Urology,Affiliated Hospital of Guilin Medical College,Guilin 541001,China; 4.Medical College,University of South China,Hengyang 421001,China;5.Second Hospital Affiliated University of South China,Hengyang 421001,China

[Abstract] Objective To study the inhibitory effect of curcumine on prostate cancer cell line PC-3. Methods By means of different concentrations(10,20,40,60,80μmol/L)of curcumin and Platinol role PC-3 cells for 24 h,by means of lente concentration (40μmol/L) of curcumin and Platinol role PC-3 cells for 4,12,24,48,72 h. MTT method was used to measure the proliferation of PC-3 cells;and detect the cell cycle changes by flow cytometry. Results The depressant effect of curcumine on PC-3 is similar to Platinol(P＞0.05),with a concentration and time-dependent. As the drug concentration and time increases,the inhibition rate increased gradually.Flow cytometry showed that,curcumine and Platinol make PC-3 cell growth arrest at G2/M phase, there was no significant difference between the two groups(P＞0.05). Each experimental group compared with the control group had significant difference(P＜0.05). Conclusion Curcumine can significantly inhibit the proliferation of PC-3 cells and has a better value of clinical application and exploitation in the treatment of prostate cance.

[Key words] Curcumine;PC-3;Proliferation;Apoptosis

姜黄素（curcumin）是从姜黄、莪术、郁金等姜科姜黄属植物的根茎中提取的一种有效成分，可溶于甲醇、乙醇、碱、醋酸、丙酮和氯仿等有机溶剂，具有抑癌、防氧化、抗炎、抗动脉粥样硬化等广泛的药理作用[1-2]。由于姜黄素的毒性小，安全性高，其抗肿瘤的作用特别受到关注，并成为现代医学天然药物研究领域的一个热点[3]。本研究观察姜黄素对激素非依赖性前列腺癌PC-3细胞的增殖抑制作用特点，为其进一步应用于临床提供实验依据。

1?材料与方法

1.1?一般材料

姜黄素粉剂购自Sigma公司，用二甲基亚砜（DMSO）溶解

成5 mmol/L，-20℃保存；人前列……