赵 磊，籍保平，王成涛

（1.北京工商大学食品添加剂与配料北京高校工程研究中心，北京100048；2.中国农业大学食品科学与营养工程学院，北京100083）

鹿茸水提物模拟胃肠消化物对小鼠脾细胞增殖的影响

赵 磊1，籍保平2，*，王成涛1

（1.北京工商大学食品添加剂与配料北京高校工程研究中心，北京100048；2.中国农业大学食品科学与营养工程学院，北京100083）

研究鹿茸水提取物模拟胃肠消化物（AEVA-SGD）的分离组分对小鼠脾淋巴细胞增殖的影响。AEVA-SGD的分离采用凝胶层析法和RP-HPLC法。应用WST-8法检测各分离组分对小鼠脾淋巴细胞增殖的影响，采用高分辨质谱对有效分离组分进行初步鉴定。结果显示，AEVA-SGD经Sephadex LH-20分离后得到6个组分（A～F）。其中，组分F对小鼠脾细胞增殖的影响最为显著，可单独促进小鼠脾细胞增殖，但对ConA诱导的T淋巴细胞增殖有抑制作用。经RPHPLC将组分F分为3个部分，初步鉴定组分F1为鸟嘌呤，F2为1-甲基鸟嘌呤和黄嘌呤，F3为多种小肽的混合物。组分F3对小鼠脾细胞增殖的影响最大，组分F对小鼠脾细胞增殖的影响是其各分离组分协同作用的结果。

鹿茸水提取物，模拟胃肠消化物，分离，小鼠脾细胞，免疫

Abstract：The aim of the present study was to investigate the effect of fractions from simulated gastrointestinal digest of aqueous extract of velvet antler（AEVA-SGD） on murine splenocytes proliferation.AEVA-SGD was fractionated by gel chromatography and RP-HPLC consecutively.The effect of each fraction on the proliferation of murine splenocytes was detected by WST-8.The effective fractions were preliminary identified by high resolution mass spectrometry.The results showed that AEVA-SGD was separated into six fractions（A～F） by Sephadex LH-20.Fraction F（Fr.F） stimulated the proliferation of resting murine splenocytes，whereas inhibited the proliferation of ConA-stimulated murine splenocytes.The effect of Fr.F on the proliferation of murine splenocytes was more effective than the other fractions.After separation by RP-HPLC，Fr.F was fractionated into three parts.The main components of each part were preliminary identified as：guanine（Fr.F1），1-methylguanine and xanthine（Fr.F2），a mixture of small peptides（Fr.F3）.Compared with Fr.F1 and Fr.F2，the effect of Fr.F3 on the proliferation of murine splenocyte was more effective.The total effect of Fr.F on the proliferation of murine splenocyte was due to the synergistic effects of its fractions.

Key words：aqueous extract of velvet antler；simulated gastrointestinal digest；separation；murine splenocyte；immunity

鹿茸为鹿科动物的雄鹿未骨化密生茸毛的幼角，与人参、灵芝、冬虫夏草和阿胶并称为中华养生五大圣品。鹿茸具有生精补髓、养血益阳、强筋健骨、延年益寿等功效，常用于功能性食品和保健品。鹿茸水提取物是鹿茸最为广泛的食用形式之一[1-2]。现代研究证实，鹿茸水提取物具有强壮机体、抗疲劳、免疫调节、促进创伤愈合、抗衰老和抗氧化等作用[3-8]。……