吕二盼，周 正，周 巍，李洋洋，张 薇，吴 涛，曾小盼，李 波，张 伟，*

（1.河北省食品质量监督检验研究院，河北石家庄 050091；2.河北农业大学食品科技学院，河北保定071001）

动物源性食品鸭血、猪血DNA提取及多重PCR鉴别研究

吕二盼1，2，周 正1，周 巍1，李洋洋2，张 薇1，吴 涛1，曾小盼1，李 波1，张 伟2，*

（1.河北省食品质量监督检验研究院，河北石家庄 050091；2.河北农业大学食品科技学院，河北保定071001）

目的：研究从鸭血、猪血中提取DNA的快速简便方法并建立多重PCR鉴别方法。方法：用KI提取法从固体块状鸭血、猪血中提取DNA，经PCR扩增检测提取效果。建立多重PCR方法鉴别动物源性食品中的鸭血、猪血成分，并对市售动物源性血制品进行检测。结果：这种方法提取到的DNA纯度较高，凝胶电泳条带整齐，背景清晰；PCR反应能扩增出目的条带。多重PCR能同时扩增出鸭和猪的条带。结论：这种改进的DNA抽提方法能获得高纯度DNA，比传统方法安全、简便、节省试剂，PCR扩增结果很好，应用多重PCR方法能同时检测出血样制品中的鸭、猪成分。

鸭血、猪血DNA，核酸提取，多重PCR检测

Abstract：Objective：Study on duck blood and pig blood aimed to find a quick and easy way to extract DNA and establish multiple PCR method for identification.Methods：The KI method was used for DNA extracting from the solid massive duck blood and pig blood and was detected by PCR amplification.A multiplex PCR method was established to identify the duck，pig blood ingredients in the food of animal original and carried on the examination to animal blood products from the market.Results：The gel electrophoresis stripes indicated that this DNA extraction method was of high purity，clear and neat.And the target bands could be amplified by PCR.Multiplex PCR simultaneously amplified duck and pig bands.Conclusion：The improved DNA extraction methods could obtain the DNA of high purity.It was safe，convenient and economical compared with the traditional method.PCR.Amplification result was good，the application of multiplex PCR method could also detect duck and pig ingredients in the blood product sample.

Key words：duck，pig blood DNA；nucleic acid extracting；multiplex PCR detect

近年来，随着疯牛病、羊瘙痒病、口蹄疫、禽流感等一些疾病在欧洲及世界各国的流行，以及一些动物源食品掺假造假现象的普遍产生，对食品、药品以及饲料产品进行动物源性成分鉴定，已经成为一个备受关注的问题[1-2]。尤其是现在时期，动物源性食品掺假、过分添加各种添加剂、使用非食用性原料和成分等各种食品安全问题不断出现，去年双汇瘦肉精事件、染色馒头事件、河南南阳毒韭菜事件等食品安全事件的频发越来越让老百姓心惊胆颤。……