中图分类号：S763.15文献标志码：A

文章编号：1673-923X（2025）07-0164-10

Analysis of the antibacterial activity of juniper essential oil against the Sphaeropsis sapinea of Pinus sylvestris var. mongolica

MATeng，HEAomen，LIAOShixian，AierpaniAbulimiti，FUSina，GUOJing，ZHOUBoru （Collegeoforestry，NortheastForestryUniversity，Harbin5oo4o，Heilongjiang，hina）

Abstract：【Objectie】TexpoeteitoryefctsadmechanissofplantessentialilsoSeropsisspinendproidinga novelandeco-friendlyapproachforcontroling thediseaseofiplodiatipblight.【Method】Themycelialgrowthate methodwassed toscreenthestyfjipealloouatsstalilsjidaeees microscopicobseatio，psiologicalandiohemicaltsting，teefetsofjuipereetaloilontemorpologyofatogenic fungalhyphaesporegeation，solublesubstanepeetratio，efeseeectiityndpathogenicityrefurthraald therebyevaluatingitsantibacterialactivitygainstSsapne.Resultheesultssoedtatamongtefourplanteentalilsth esentialilofjipradtestibitoryectotemyelialgrowtofSsapinenditalsodabroadatibacteralstru. significantly inhibitingS.sapinea growth withan inhibitionrateof 87.55% at 40mL/L .Spore germination was notably suppressed at concentrations above 5mL/L .Microscopic observations revealed that juniper essential oil caused mycelial shrinkage，rupture，and swelling.Further studies showed that after 12 hours of treatment， extracellular soluble protein levels in the EC₃₀ and EC₅₀ treated groups were4.36timesnd5.9tieshighertanthotrol，espectivelydcatingatjuipersetialoildsuptedtheinteityof pathogen’scellmembranestructure.Treatedmyceliaaccumulatedlrgeamountofreactiveoxygenspecies，andPIandFDAstaiing resultsndicatedthatjuniperessentialoilsignificantlyeducedthecellviabilityofSsapineaEnzyeactivityassysrevealedthat after9hoursoftreatment，teactivitiesofsuperoxidedismutase（SOD），peroxidase （POD）andcatalase（CAT）begatodcreasehe activities of endo ⋅β⋅ -1，4-glucanase （EG）， polygalacturonase （PG），and pectin lyase （PL） gradually decreased and remained consistently lower than the control group.After 12 hours of treatment with EC₃₀ and EC₅₀ ，theenzyme activitieswere 65.54% and 76.51% （Plt; 0.05）， 62.8% and 79.2% and 82.62% and 92.55% lower than the control group，respectively. These results indicate that juniper essential oil significantly affects the stability of the enzyme system within S. sapinea，induces oxidative stress responses，and reduces itsinfectivity and pathogenicity.【Conclusion】Juniper essential oil effectively inhibits the growthof S. sapinea，disrupts itsstructure，and reducesits pathogenicity.Thisstudyprovidesatheoreticalbasis forthebiologicalcontrolofDiplodia tipblight.

words：jniperstialil;inussestrisamonglica;Dipldiatiplight;Serosisintibactaltii

樟子松Pinussylvestrisvar.mongholica是一种优良的造林树种，树形美观、材质较好，在水土保持、防风固沙、环境绿化及生态建设中至关重要。在樟子松生长发育过程中，易遭受松球壳孢菌Sphaeropsissapinea（异名：Diplodiasapinea）的侵染，引起枯梢病，导致树势衰弱。由于该病害是一种传染性病害，必须加以防范[1。目前，樟子松枯梢病防治多为化学防治，虽能取得一定成效，但其破坏环境、对人体致癌及部分国家法律限制等影响且长期使用化学药剂易出现抗药性菌株[2]。因此，迫切需要筛选开发安全、高效杀菌剂应用于樟子松枯梢病防治。

近年来，植物精油作为植物源杀菌剂研究及应用备受关注。植物精油是一类从植物叶、种子、花和根茎等部位提取的植物次生代谢产物。其具有广谱抑菌性，毒性较小，绿色天然，无药物残留，可降解，对环境和动物相对安全[3-4]。因此，其在抑菌抗菌上的作用越来越受关注，被认为是最有潜力且更安全的绿色抗菌剂之一，已被广泛研究作为化学农药的替代品[5]。据报道，一部分精油对抑制植物病原菌生长及病害防控方面具备良好的应用前景，如牛至、香茅、丁香精油三种植物精油对白色葡萄球菌Staphylococcusalbus、白色念珠菌Candidaalbicans、金黄色葡萄球菌S.aureus等5种常见病原微生物具有一定抑菌作用[。卢彩会等[报道，姜黄油对沙门氏菌Salmonella、枯草芽孢杆菌Bacillussubtilis、蜡样芽孢杆菌B.cereus及藤黄八叠球菌Sarcinalutea均有抑制效果。高原等[8研究发现丁香精油对金黄色葡萄球菌S.aureus和铜绿假单胞菌Pseudomonasaeruginosa具有明显的抑制作用。……