中图分类号：S794.4 文献标志码：A

Study on the correlation between selenium enrichmentability of Camellia oleifera seedlings and soil selenium forms based on phosphorus selenium combination application

ZHAO Xueyan， TANG Yu， CHEN Zhuoling，ZHANG Xueling

（aJiangxiKeyLaboratoryofSubropialFortResoucsCulivation;b.KyLaboatoyoforestEosteProtectionandstoati thePoyangeatestasito

Abstract：【Obective】ToaalyetheseleniumcontentinvariousorgansofC.oleiferasedlings，aswellasthetotalslenuote anditsccurrencefomsisoil，toexploeteorelationbteenteeleniumecmentabityof.oleifraseedligsadsoil seleniumforsndtoprovideteoreticalrefereneanddatasupportforselenumrichultivatioofC.fera.【Method】egafted seedlings of C. oleifera‘Ganwu 2^， were used as test materials，through three levelsof phosphorus，P1 （30 mmol/L），P2（60 mmol/L）， P3 （90mmolL ，fourlevels of selenium， Se0（ 0.00mmol/L ，Se 1（0.04mmolL） Se2 0.08mmolL ）， Se3（0.12mmolL ），atotalof 12treatmentsachtreatmenthad4replicates，4pantsintecontrolgroupCK），atotalof52testplants.efrtilzatiotiewas earlyApril（April6th）），arlyMay（May6th），andthesamplingtimewasJuly（July5th）.Tecontetsofseleniuminrots，ste leaves of C oleifera seedlings were determined by inductively coupled plasma massspectrometry.The contents of total selenium， water-solubleslenium，exchangeableselenum，oxidizableseleum，educibleselenumandresidalselenuminsoilwredteind byatomicfuorescencespectrometry.【Result】1）SeleniumcontentandenrchmentcoeficientofvariousorgansofC.oleifera：the root of C oleifera seedlings reached the highest value of 36.07mg/kg under P3Se3 treatment，and the stems and leaves of C.oleifera seedlingsreachedthepeakvalueof7.05and 5.78mg/kg underP2Se3 treatment，respectively;on the whole， the seleniumcontent of each organ of C oleifera seedlingswas ranked as follows：root gt; stem gt; leaf.Under the concentration of P3SeO，the enrichment ability ofseleniuminrootswasstronger.UnderthetreatmentofPSeO，theenrichmentabilityofselenuminstemswasbeter.Underthe treatment ofP2SeO，theenrichment abilityofseleniuminleaves wasbeter;2）Total seleniumandseleniumspeciationcontent root soil：Water-solubleseleniumandweaklyacid-extractableseleniumreachedtheirpeakunderP3Se3treatment.Bothfomsofelenium contentgraduallyincreased withtheincreaseandaccumulationofSeoncentration，whilemaintainingaonstantPconcentration; 3）Thecorrelation between total selenium in plantsand soil，and soil selenium formed： The root of C oleiferaseedlingswassignificantly positively correlated with exchangeable selenium （ （Plt;0.01） ; The stem of C oleifera seedlings was significantly positively correlated withexchangeableselenium （Plt;0.01） ）.Therewasa significant positivecorrelation between leafandwater-soluble selenium （Plt;0.05） L and a very significant positive correlation with exchangeable selenium （Plt;0.01 ）.The total selenium in soil was significantly positively correlated with water-soluble selenium （Plt;0.05 ）andhighly significantly positively correlated with four formsof selenium，including exchangeable selenium （Plt;0.01） .【Conclusion】 Under the treatment of P2Se3 （P concentration： 60mmol/L ，Se concentration： 0.12mmolL） ，theabsorption effect of seleniumcontent in various organs of C oleifera seedlingswasgood，indicatingastrongselenium enrichmentabilityThissuggeststhatinthecombinedaplicationofphosphorusandseleniumfertliers，phosphorusfertilizercan effectively promote selenium absorption and increase the selenium content of plants.The root of C. oleifera seedlingswas significantly positively correlated with exchangeable selenium （Plt;0.01 ）.Thestemand leaf partsof C oleifera seedlingswere also significantly positively correlated with exchangeable selenium （Plt;0.01 ）.Overall，there isa significant positive correlation （Plt;0.05 ）between the seleniumcontent in the roots，stems，and leaves of C oleiferaseedlingsand theavailable seleniumin thesoil.

Keywords： C.oleiferasedlings;appicationofphosphorusandselenincombination;selenumerichmentablityselenufos; correlation

硒（Se）是天然存在的非金属元素，是人类、动物以及微生物必需的微量元素，它参与人体中的抗氧化防御系统和调节激素平衡等过程[。缺硒会加速身体衰老，并可能导致癌症、心血管等疾病[2-3]。适当补充硒可以提高人体抵抗某些疾病的能力[46]。在多种补硒方式中，通过饮食补充硒是一种安全、可行的方法[，以植物性食物（以硒氨基酸为主）补硒安全有效且具有生物效价[8-9]。硒对植物的生物有效性及植物对硒的吸收累积量不仅取决于土壤中硒的总量，与硒的赋存形态也密切相关[0]。硒在土壤中以多种形态存在[]，瞿建国等[12]利用不同浸提剂分步骤连续浸提的方法将土壤中的硒划分为5种形态。磷是植物生长必需的营养元素，在植物生长、代谢、产量和品质形成中发挥着不可替代的作用[13]。而江西土壤为酸性红壤，普遍缺磷[14]，缺磷会影响植株对硒元素的吸收，即使是在富硒区域，植物中硒的有效性也可能很低。磷与硒虽为不同族元素，但都以阴离子形态被土壤胶体吸附，两者之间的化学行为具有一定的相似性，富硒土壤中多为亚硒酸盐[15]，亚硒酸盐和磷酸盐在土壤颗粒表面具有相似的吸附位点[1，存在竞争吸附关系[17]。安军妹等[8]和祝姣姣等[19]研究指出，磷肥与亚硒酸盐配施可促进土壤中的硒的形态转化，提高植株对硒的吸收和积累。

油茶Camelliaoleifera隶属于山茶科Theaceae山茶属Camellia，是我国特有的纯天然高级木本油料[20]，是我国主要的经济林树种之一，占全球油茶资源的 95% 以上[21]，生命周期长[22]，极具营养、健康、经济和社会价值[23]。……