中图分类号：Q945.79 文献标识码：A 文章编号：1007-0435（2025）07-2388-12

引用格式：，等.植物根际促生菌（PGPR）对高寒区3种栽培饲草促生效果研究[J].草地学报，2025，33 （7） ：2388-2399 LEI Sheng-yan，ZHANG Ying，SU Bei-bei，et al.Effects of Plant Growth Promoting Rhizobacteria （PGPR）on Three Cultivated Forages in Alpine Area[J].Acta Agrestia Sinica，2O25，33（7） ：2388-2399

Effects of Plant Growth Promoting Rhizobacteria（PGPR）on Three Cultivated Forages in Alpine Area

LEI Sheng-yan¹，ZHANG Yingl，SUBei-bei1，DAO Ri-na¹，MALin-xiong1，TIE Xiao-longl，LIUWen-hui² （1.CollegeofgiludialHusbandryQigiUivesityKybotoryofiGassadEoloji Xining，QinghaiProvince81Oo16，China；2.LaboratoryofGermplasmResourcesResearchandUtilizationofQinghai-TibetPlateau， Academy of Animal Scienceand Veterinary Science，Xining，Qinghai Province 8lOol6，China）

Abstract：In order to investigate the growth promotion effects of 5 strains of azotobacter and 5 strains of phospho rus solubilizing bacteria on 3 kinds of cultivated forages in high cold area，14 groups of single and compound inoculants were used to inoculate com （Zea mays L.），oat （Auena sativa L.） and alfalfa（Medicago Satiua L.），and plant height，biomass and root morphology were determined in this study.The results showed that there were diffrences in the growth indices ofcorn，oat and alfalfa. Among them，compound inoculant A+B+ （204号 F had significant growth promoting efects on aboveground biomass，underground biomass，root total length and root surface area ofcorn，which were significantly higher than those on other treatments （ Plt;0.05） .Compound inoculants B+G+H and A+B+F had obvious growth-promoting effects on plant height，aboveground biomass，underground biomass，root length，root diameter，root volume and root surface area of oat. In the alfalfa inoculation experiment， the effects of compound inoculant A+F on plant height，aboveground biomass，under ground biomass，root length，root diameter，root volume and root surface area of alfalfa were significantly higher than those on other treatments and controls （Plt;0.05） .In short，the compound inoculant had more potential to develop microbial fertilizer than the single strain，which could providea new idea for applied agriculture. Key words：Plant growth promoting rhizobacteria;Alpine area; Cultivated forage; Promoting effect

植物根际促生菌（Plant growth promoting rhizobacteria，PGPR）是指定殖于植物根系或植物茎叶的，生活在土壤中的一类有益微生物，它能够促进植物生长发育、增加作物产量以及防治植物病虫害等[1-2]。PGPR的促生机理主要包括固氮、溶磷、解钾、溶铁、分泌植物激素及调节相关物质和释放挥发性物质等改变根际区域微生物的丰度[3]。PGPR的促生作用分为两类，一类是直接作用，另一类是间接作用。直接作用是促进植物吸收利用微量营养元素，如固氮、解钾、溶磷和溶铁等，调节植物激素水平如吲哚乙酸，IAA、赤霉素，GA、细胞分裂素，CTK等来促进植物生长；间接作用是抑制病原微生物，减少植物病害和增强植物的抗逆性[4]。大气中N₂ 含量有 78% 之高，但它被高度惰性的植物能直接利用的几乎为零，而氮是植物生长所必需的元素之一。固氮微生物作为促生菌的一种，可以通过生物固氮的过程（Biological nitrogen fixation，BNF）可以将大气中游离的氮转化为氮化合物，供植物生长利用5-6。磷元素在土壤中以不可溶的形式存在，也是植物生长所必需的元素之一，主要以矿物质形式和有机形式存在，通常不易被植物吸收利用。同样溶磷菌也是植物根际促生菌的一种，可以通过释放有机酸以溶解磷酸钙和岩石中的磷，来供给植物可利用的磷元素[8-9]。葡宝珺等[10]从青海省高寒草甸根际土中分离筛选了4株优良的溶磷菌株，通过盆栽试验，结果表明4株溶磷菌株对披碱草的株高和地上干重均有显著的促进作用。……