李新淼 王圆圆 Heshuote MAILISI 白玉廷 包音都古荣·金花 呼格吉勒图 敖日格勒 侯荣伦 薛强

摘 要：随机采集48 月龄安格斯和西门塔尔母牛各37 头血液样品以及用于肉质分析的各5 头母牛背最长肌肉样。样品经过前处理和甲酯化后，利用气相色谱-质谱联用仪测定脂肪酸组成和含量，对血液和肌内脂肪酸组成特性进行分析。结果表明：安格斯和西门塔尔母牛血液和肌内脂肪中均测得36 种脂肪酸，样品中均含有51%以上的高含量饱和脂肪酸（saturated fatty acids，SFA），且具有以棕榈酸和硬脂酸为主的组成特点，二者总含量分别约占血液和肌内脂肪SFA的92%和89%;不饱和脂肪酸（unsaturated fatty acids，UFA）中油酸相对含量最高，其次为亚油酸;血液和肌内脂肪的油酸含量均约占单不饱和脂肪酸总量的87%，UFA中各主要脂肪酸组成在血液中有显著性差异，而在肌内脂肪中无显著性差异;血液和肌内脂肪中n-6/n-3 PUFA均符合联合国粮农组织（United Nations Food Agriculture Organization，FAO）推荐比例（5～10），血液中P/S高于世界卫生组织（World Health Organization，WHO）推荐标准（≥0.4）。综上所述，高寒草原饲养的纯种安格斯和西门塔尔母牛血液和肌内脂肪酸组成特性相似，UFA符合FAO和WHO理想比值及推荐标准，具有较高的营养价值。

关键词：高寒草原饲养;安格斯母牛;西门塔尔母牛;血液脂肪酸;肌內脂肪酸

Blood and Intramuscular Fatty Acid Composition of Angus and Simmental Cows in Alpine Grassland

LI Xinmiao1，2， WANG Yuanyuan1，2， Heshuote MAILISI3， BAI Yuting4， BAOYINDUGURONG·Jinhua1，2，*，

HUGEJILETU5， AORIGELE5， HOU Ronglun5， XUE Qiang5

（1.College of Food Science and Engineering， Inner Mongolia Agricultural University， Hohhot 010018， China; 2.Inner Mongolia Key Laboratory of Biomanufacturing， Hohhot 010018， China; 3.The Fu Foundation School of Engineering and Applied Science， Columbia University， New York 10027， USA; 4.College of Veterinary Medicine， Inner Mongolia Agricultural University，

Hohhot 010018， China; 5.Inner Mongolia Hesige Green Industry Import and Export Co. Ltd.， Xilinguole 026000， China）

Abstract： Blood samples from 37 randomly selected Angus and Simmental cows at the age of 48 months each and Longissimus dorsi muscle samples from 5 cows each breed were collected for this study. After pretreatment and methyl esterification， the composition of fatty acids in blood and muscle was investigated by gas chromatography-mass spectrometry （GC-MS）. The results showed that 36 fatty acids were detected in blood of each breed and also in intramuscular fat. All samples had high contents （up to more than 51%） of saturated fatty acids （SFA）， which were mainly composed of palmitic acid and stearic acid， accounting for 92% and 89% of the total SFA in blood and intramuscular fat， respectively. In addition， oleic acid was the most predominant unsaturated fatty acid （UFA）， followed by linoleic acid; the former accounted for about 87% of the total monounsaturated fatty acids in blood and intramuscular fat， respectively. The major UFAs showed significant differences in blood but not in intramuscular fat. Furthermore， n-6/n-3 PUFA ratio in blood and intramuscular fat were within the United Nations Food Agriculture Organization （FAO） recommended range （5–10）， and blood P/S ratio was higher than the World Health Organization （WHO） recommended standard （≥ 0.4）. This study concluded that the composition of blood and intramuscular fatty acids was similar between pure Angus and Simmental cows raised in alpine pasture and UFA composition accorded with the ideal pattern recommended by the FAO and WHO， suggesting high nutritional value.

Keywords： alpine grassland feeding; Angus cows; Simmental cows; blood fatty acids; intramuscular fatty acids

DOI：10.7506/rlyj1001-8123-20210514-131

中图分类号：S823.92                                        文献标志码：A 文章编号：1001-8123（2021）08-0042-06

引文格式：

李新淼， 王圆圆， Heshuote MAILISI， 等. 高寒草原地区安格斯和西门塔尔母牛血液及肌内脂肪酸组成特性[J]. 肉类

研究， 2021， 35（8）： 42-47. DOI：10.7506/rlyj1001-8123-20210514-131.    http：//www.rlyj.net.cn

LI Xinmiao， WANG Yuanyuan， Heshuote MAILISI， et al. Blood and intramuscular fatty acid composition of Angus and Simmental cows in alpine grassland[J]. Meat Research， 2021， 35（8）： 42-47. DOI：10.7506/rlyj1001-8123-20210514-131.

http：//www.rlyj.net.cn

随着经济的发展和人们生活水平的提高，居民膳食结构不断调整改善，牛肉的消费量逐年增加，市场上优质牛肉供不应求[1-4]。……