苏馨 车天宇 谢遇春 赵存 郭俊涛 王志新 包永泉 李金泉 刘志红

摘 要：挑选内蒙古地区相同饲喂条件下的乌珠穆沁羊、杜泊羊和萨福克羊各6 只，取背最長肌，通过气相色谱-质谱进行脂肪酸检测，构建指纹图谱，利用中药色谱指纹图谱相似度评价系统软件进行指纹图谱相似度分析和主成分分析。结果表明：杜泊羊和萨福克羊背最长肌脂肪酸指纹图谱相似度为0.991，杜泊羊和乌珠穆沁羊背最长肌脂肪酸指纹图谱相似度为0.987，萨福克羊和乌珠穆沁羊背最长肌脂肪酸指纹图谱相似度为0.994。3 种羊背最长肌脂肪酸指纹图谱中共存在12 个共有峰，以硬脂酸（C18：0）色谱峰为参比峰，对12 个共有峰的保留时间和相对峰面积进行主成分分析，可准确鉴别以上3 个品种羊。

关键词：脂肪酸;指纹图谱;乌珠穆沁羊;杜泊羊;萨福克羊;相似度

Fatty Acid Fingerprinting of Wuzhumuqin， Dorper and Suffolk Sheep

SU Xin1， CHE Tianyu1， XIE Yuchun1， ZHAO Cun1， GUO Juntao1， WANG Zhixin1， BAO Yongquan2， LI Jinquan1，*， LIU Zhihong1，*

（1.The Inner Mongolia Autonomous Region Goat Genetics and Breeding Engineering Technology Research Center， Key Laboratory of Mutton Sheep Genetics and Breeding， Ministry of Agriculture and Rural Affairs， Key Laboratory of Animal Genetics， Breeding and Reproduction in Inner Mongolia Autonomous Region， College of Animal Science， Inner Mongolia Agricultural University，

Hohhot 010018， China; 2. Eastern Wuzhumuqin Animal Disease Prevention and Control Center， Xilingol 026000， China）

Abstract： In this experiment， six Wuzhumuqin， Dorper and Suffolk sheep raised under the same conditions in Inner Mongolia each were selected and slaughtered. Longissimus dorsi muscles were taken for fatty acid fingerprinting by gas chromatography-mass spectrometry （GC-MS）. Similarity analysis of the developed fingerprints was carried out using the software of similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine （TCM） and principal component analysis （PCA） was performed as well. The results showed that the similarity in the fatty acid fingerprint of Longissimus dorsi was 0.991 for Dorper versus Suffolk sheep， 0.987 for Dorper versus Wuzhumuqin sheep， and 0.994 for Suffolk versus Wuzhumuqin Sheep. A total of 12 peaks were common to the fatty acid fingerprints of Longissimus dorsi of the three breeds. Based on the retention times of the 12 common peaks and the relative peak areas， PCA accurately discriminated these breeds using stearic acid （C18：0） as the reference peak.

Keywords： fatty acids; fingerprint; Wuzhumuqin sheep; Dorper sheep; Suffolk sheep; similarity

DOI：10.7506/rlyj1001-8123-20210312-068

中图分类号：TS251.2                                   文献标志码：A 文章编号：1001-8123（2021）08-0037-05

引文格式：

苏馨， 车天宇， 谢遇春， 等. 乌珠穆沁羊、杜泊羊和萨福克羊脂肪酸指纹图谱的建立[J]. 肉类研究， 2021， 35（8）： 37-41. DOI：10.7506/rlyj1001-8123-20210312-068.    http：//www.rlyj.net.cn

SU Xin， CHE Tianyu， XIE Yuchun， et al. Fatty acid fingerprinting of Wuzhumuqin， Dorper and Suffolk sheep[J]. Meat Research， 2021， 35（8）： 37-41. DOI：10.7506/rlyj1001-8123-20210312-068.    http：//www.rlyj.net.cn

气相色谱-质谱指纹图谱联用技术是将样品质谱图进行相似性分析，将具有代表性的图谱进行提取，以为后期样品掺假鉴别和产地溯源提供帮助，该方法在肉品中应用较少，但在药品及其他食品中应用较为广泛。陈学敏等[1]通过顶空固相微萃取结合气相色谱-质谱联用法研究欧拉羊肉熟制时的挥发性风味物质，并构建相应指纹图谱。Han Jingtao等[2]通过气相色谱技术确定4 种蟹中7 种特征性脂肪酸，建立我国4 种主要经济蟹的脂肪酸指纹图谱。……