无水保活运输温度对史氏鲟氧化应激的影响
2021-09-05周文白婵王炬光柴毅鉏晓艳廖涛熊光权
周文 白婵 王炬光 柴毅 鉏晓艳 廖涛 熊光权



摘 要:為了提高史氏鲟的保活时间和存活率,选取合适质量浓度MS-222麻醉史氏鲟,研究不同无水保活运输温度(4、8、12 ℃)对史氏鲟氧化应激的影响,分别记录模拟保活运输12 h和24 h后各温度组的存活率,分析保活运输前后应激指标和抗氧化指标变化。结果表明:MS-222麻醉史氏鲟,较佳镇静质量浓度为100 mg/L、镇静时间为(2.1±0.1) min,较佳麻醉质量浓度为110 mg/L、麻醉时间为(2.1±0.3) min;保活运输12 h时,12 ℃条件下存活率最高,为85%,保活运输24 h时,4 ℃条件下存活率最高,为62.5%;保活运输12 h后12 ℃条件下血糖浓度和皮质醇含量显著低于其他组,超氧化物歧化酶和谷胱甘肽过氧化物酶(glutathione peroxidase,GPX)活性显著低于其他组,同时丙二醛(malondialdehyde,MDA)含量最低(P<0.05),表明受到的应激最小;保活运输24 h时,各温度组过氧化氢酶活性无明显差异,4 ℃条件下GPX活性较低,同时MDA含量显著低于其他组(P<0.05)。因此在史氏鲟短时间(12 h)保活运输时,较高温度(12 ℃)减少应激效果明显,是较适的保活温度;在较长时间(24 h) 保活运输时,低温能更好保持低代谢和麻醉状态,4 ℃是较适的保活温度。
关键词:MS-222;史氏鲟;温度;无水保活运输;氧化应激
Abstract: In order to improve the survival time and survival rate of Amur sturgeon, the fish were anaesthetized with an appropriate concentration of MS-222 to explore the effects of different temperatures (4, 8 and 12 ℃) during waterless live transportation on oxidative stress in it. The survival rate of the fish was recorded after 12 and 24 h of simulated live transportation, and the changes in oxidative stress and antioxidant indicators were analyzed before and after live transportation. The results showed that the appropriate sedative concentration of MS-222 was 100 mg/L and the sedation time was (2.1 ± 0.1) min; the appropriate anaesthetic concentration was 110 mg/L and the anesthesia time was (2.1 ± 0.3) min. After 12 h live transportation, the highest survival rate of 85% was observed at 12 ℃. After 24 h live transportation, the highest survival rate of 62.5% was observed at 4 ℃. After 12 h live transportation, blood sugar, cortisol and malondialdehyde (MDA) levels as well as the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) were significantly lower in the 12 ℃ group than in the other temperature groups (P < 0.05), indicating that the lowest level of oxidative stress occurred in the 12 ℃ group. After 24 h live transportation, there were no significant differences in catalase (CAT) activity among all groups. GPX activity was lower in the 4 ℃ group, and MDA content was significantly lower in this group than in the other groups (P < 0.05). Therefore, when the sturgeon was transported without water for a short time (12 h), a significantly lower level of oxidative stress was observed at higher temperature (12 ℃), suggesting that the appreciate survival temperature was 12 ℃. When the sturgeon was transported for a long time (24 h), low metabolism and anesthesia were maintained better at lower temperature (4 ℃), so that the fish could survive better.
Keywords: tricaine methanesulfonate (MS-222); Acipenser schrenckii; temperature; waterless live transportation; oxidative stress
DOI:10.7506/rlyj1001-8123-20210408-095
中图分类号:TS254.4 文献标志码:A 文章编号:1001-8123(2021)07-0032-06
引文格式:
周文, 白婵, 王炬光, 等. 无水保活运输温度对史氏鲟生化指标的影响[J]. 肉类研究, 2021, 35(7): 32-37. DOI:10.7506/rlyj1001-8123-20210408-095. http://www.rlyj.net.cn
ZHOU Wen, BAI Chan, WANG Juguang, et al. Effects of different temperatures during waterless live transportation on oxidative stress in Amur sturgeon (Acipenser schrencki)[J]. Meat Research, 2021, 35(7): 32-37. DOI:10.7506/rlyj1001-8123-20210408-095. http://www.rlyj.net.cn
史氏鲟(Acipenser schrencki)隶属于鲟形目、鲟亚科,被认为是现存最古老的生物之一,具有很高的经济和营养价值,近几十年来,产量逐渐增加[1]。中国是鲟鱼产量最大的国家,在世界上占有很大的比重,2010—2017年,其产量占世界鲟鱼产量80%左右[1]。活鱼在中国特别受欢迎,因为在中国文化中,新鲜杀死的鱼比死鱼更美味[2-4]。如今,高价值品种的活鱼销量大幅增长,其销售价格比大宗淡水鱼高5~10 倍,带来了可观的经济效益[5]。为了提高利润率和口感质量,鲟鱼通常是鲜活销售[6-7]。然而,活鱼运输是一个相对复杂的过程,需要更好地控制潜在的运输刺激,防止鱼的生理应激反应,否则过度的生理应激会导致鱼的活力下降,甚至死亡[8-9]。……
