冯伟 冯雅琪 徐晶雪

摘 要：乳酸菌是生物体中有益菌的重要组成，筛选出产酸能力强、能够起到肠道医生作用且安全性高的乳酸菌，并应用于相关发酵产品生产对生物健康至关重要。该研究通过碳酸钙-MRS透明圈法从酸菜中筛选乳酸菌，利用对羟基联苯法测定乳酸菌发酵液中乳酸含量，选取产乳酸超过30mg/mL的菌株4株作为候选菌株，对其进行常见致病菌的抑菌能力和十大类常见抗生素的药物耐受性的测定。结果表明，备选菌株对常见致病菌均具有一定的抑菌性能，其中2个菌株在抗生素耐药性方面表现出了较好的安全性。筛选出的2株菌株的产酸性能强，肠道有害菌抑菌效果明显，安全性高。

关键词：乳酸菌;乳酸;抑菌;耐药

中图分类号 TS255.54文献标识码 A文章编号 1007-7731（2021）07-0032-03

Screening of Functional Lactic Acid Bacteria with High Lactic Acid Production in Kimchi

FANG Wei1 et al.

（1Daqing Normal University， Daqing 163712， China）

Abstract： Lactic acid bacteria are an important component of beneficial bacteria in organisms. It is very important for biological health to screen the lactic acid bacteria with strong acid production ability， which can play the role of intestinal doctor and have high safety and apply them to the production of related fermentation products. This experiment through the calcium carbonate-MRS transparent ring method from the screening of lactobacillus in the sour pickled cabbage， using the method of hydroxy diphenyl lactic acid content in the determination of lactic acid bacteria fermented liquid， and choose to produce lactic acid more than 30mg/mL strains 4 strains as candidate strain， on the strains of common pathogenic bacteria of ten kinds of common antibiotics antibacterial ability and the determination of drug tolerance. The results showed that all the candidate strains had certain antibacterial properties against common pathogenic bacteria， and two strains showed good safety in antibiotic resistance. Finally， two strains were obtained， which had strong acid production ability， obvious inhibitory effect on intestinal harmful bacteria and high safety.

Key words： Lactobacillus; Lactate; Antibacterial; Drug resistance

1 引言

隨着经济的飞速发展，人们更加注重健康养生。乳酸菌是人体肠道有益菌群中的重要组成部分，近年来，随着人们对乳酸菌功能和作用机理的深入研究，乳酸菌被认为是最具有开发价值的健康菌，越来越受到关注。筛选出品质和性状更为优异的菌种对于乳酸菌产品的研发尤为重要。

生物肠道有益菌中乳酸菌数量多，可通过菌体自身和产生的代谢物抑制致病菌从而调节肠道菌群平衡。乳酸菌在生物肠道内可以利用其中的营养物质代谢产生大量乳酸，乳酸使肠道酸性环境加强，许多致病菌因不能耐受酸性环境而死亡，也能通过产生酶阻止致病菌及其毒素黏附和侵入肠黏膜上皮细胞。……