雷廷 李敏 李荣荣 程璟侠

摘 要：为阐明CYP4家族基因在温带臭虫（Cimex lectularius）解毒代谢机制中的作用，应用生物信息学软件对温带臭虫CYP4C1蛋白的结构与生物学特性进行预测和分析。通过GenBank数据库获得温带臭虫CYP4C1基因与CYP4C1蛋白序列信息，利用生物信息学软件对CYP4C1基因、CYP4C1蛋白进行分析;并构建系统发育树。结果表明，温带臭虫CYP4C1的cDNA编码区全长为1053bp，编码500个氨基酸;第5～23位氨基酸为疏水区;不稳定指数为37.85;在5～27位氨基酸之间形成1个典型的跨膜区;无信号肽结构;亚细胞定位在细胞质中;具19个磷酸化位点，3个糖基化位点以及4个N-酰基化位点;二级结构预测显示，主要结构元件为α-螺旋和无规卷曲;含3个二硫键;含P450结构功能域。

关键词：温带臭虫;CYP4C1蛋白;结构;功能;生物信息学

中图分类号 R384文献标识码 A文章编号 1007-7731（2021）07-0018-06

Bioinformatics Analysis of CYP4C1 Protein in Cytochrome P450 of Cimex lectularius

LEI Ting1 et al.

（1Department of Biology， Taiyuan Normal University， Jinzhong 030031， China）

Abstract： To illuminate the foundation for elucidating the role of CYP4 family genes in the detoxification and metabolism of Cimex lectularius， bioinformatic softwares were applied to predict and analyze the structure and biological characteristics of the CYP4C1 protein. The sequence information was obtained from GenBank database. CYP4C1 and CYP4C1 protein were analyzed by bioinformatics software. And the phylogenetic tree was conducted. The results showed that the full-length of CYP4C1 cDNA is 1053 bp long， encoding 500 amino acids; the predicted hydrophobic region is 5-23 and the instability index is 37.85; the transmembrane region is 5 to 27; it has no signal peptides and the subcellular localization analysis indicates it exists in cytoplasm; .it contains 19 phosphorylation site， 3 glycosylation sites and 4 N-acylation sites; its secondary structure is composed mainly of α-helix and random coil; protein domain prediction shows that it contains the P450s structural domain.

Key words： Cimex lectularius; CYP4C1 protein; Structure; Function; Bioinformatics

温带臭虫（Cimex lectularius）是隶属于半翅目（Hemiptera）臭虫科（Cimicidae）臭虫属（Cimex）的一类无翅昆虫，与人类密切相关，主要靠吸食人血为生，昼伏夜出，叮咬人体的皮肤后，可以致使部分皮肤受到损伤，出现红肿、瘙痒等症状，部分人可能发生激烈的过敏反应，严重者会出现丘疹样荨麻疹[1]。温带臭虫在全世界范围内均有分布，近十几年来，温带臭虫在欧洲、北美、澳大利亚和亚洲等部分发达国家和地区重新出现[2-4]。我国部分地区，温带臭虫也日趋常见，有广泛发生的风险[5]。现阶段，臭虫防治的主要方法是喷洒化学杀虫剂，然而随着杀虫剂的大量应用，大多数臭虫对不同的杀虫剂有了明显的抗性[6-7]，使得臭蟲的防治进入新阶段。……