陈莉菁 刘子记 谢尚潜 凌鹏 朱婕

摘  要：番茄是世界上最重要的蔬菜作物之一，病害的发生和蔓延使番茄生产受到严重影响，培育多抗番茄品种是防治病害最为经济和有效的方法。本研究利用功能标记对234份番茄材料进行Cf-9、Mi-1、I-2、Ph-3和Tm-2a等5个抗病基因的检测，发现其中含Cf-9的番茄材料为136份，含Mi-1的材料为20份，含I-2的材料为124份，含Ph-3的材料38份，含Tm-2a的材料为24份。234份番茄材料中，不含这5个抗病基因的材料有29份;含1个抗病基因的材料有106份;含2个抗病基因的材料有70份;含3个抗病基因的材料有23份;含4个抗病基因的材料有6份，分别为11CT387-2M、11CHT164-2、11CT150、11CT271、11CT730和11CT774;同时含这5个抗性基因的番茄材料尚未检测到。该研究结果将为进一步开展多个抗病基因的聚合育种提供参考依据。

关键词：番茄;功能标记;抗病基因;聚合育种

中图分类号：S641.2      文献标识码：A

Detection of Five Disease Resistance Genes in 234 Tomato Materials with Functional Markers

CHEN Lijing1，2， LIU Ziji3， XIE Shangqian2， LING Peng2， ZHU Jie1，2

1. College of Horticulture， Hainan University， Haikou， Hainan 570228， China; 2. Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants （Hainan University）， Ministry of Education， Haikou， Hainan 570228， China; 3. Tropical Crops Genetic Resources Institute， Chinese Academy of Tropical Agricultural Sciences， Haikou， Hainan 571101， China

Abstract： Tomato is one of the most important vegetable crops in the world. Tomato production is seriously affected by the occurrence and spread of diseases. It is the most economical and effective way to control diseases by cultivating varieties with multiple resistant genes to different diseases. Functional markers were used to test the appearance of Cf-9， Mi-1， I-2， Ph-3 and Tm-2a genes in 234 tomato materials. Cf-9 was found in 136 materials. Mi-1 was found in 20 materials. I-2 was found in 124 materials. Ph-3 was found in 38 materials. And Tm-2a was found in 24 materials. Among the 234 tomato materials， 29 did not contain any of these five resistant genes， 106 contained one resistant gene， 70 contained two resistant genes， 23 contained three resistant genes， 6 contained four resistant genes （line 11CT387-2M， 11CHT164-2， 11CT150， 11CT271， 11CT730 and 11CT774）. None of the materials had five resistant genes. The results would lay a foundation for resistance genes pyramiding in the future.

Keywords： tomato; functional marker; resistance gene; multiple genes pyramiding

DOI： 10.3969/j.issn.1000-2561.2021.06.025

番茄（Solanum lycopersicum L.）色彩鮮艳，营养丰富，既可作为蔬菜，又可作为水果食用，且加工潜力较大，是世界上经济效益最高、栽培最广泛的园艺作物之一。番茄是典型的设施栽培作物，极易受到设施的特殊环境、连作障碍及病害的影响。迄今为止已发现的番茄病害达200多种，其中较严重的病害包括40余种，极大影响了番茄的产量和质量[1]。因此，选育抗病性强的番茄品种显得尤为关键。迄今为止，在番茄中定位并克隆了一批抗病基因，包括番茄烟草花叶病毒病、叶霉病、晚疫病、枯萎病和根结线虫病抗性基因等。

番茄叶霉病由半知菌（Cladosporium fulvum）引起。目前发现的叶霉病抗性基因至少有24个（Cf-1至Cf-24）。……