张月 袁媛 何弦 王宇婷 吕美玲 伍炳华 陈清西

摘  要：基于茉莉花叶转录组（GenBank登录号为GHOY00000000）筛选了2个在花瓣中及夜间优势表达，且与其他物种中香气调控转录因子同源度高的MYB基因进行克隆并分析了它们在花朵开放中的表达模式。2个MYB基因编码序列长度分别为864 bp和588 bp，编码287 aa和195 aa个氨基酸残基。分别与拟南芥AtMYB108及AtMYB24亲缘关系相近，与油橄欖（Olea europaea var. sylvestris）MYB108-like protein和MYB305-like protein相似度最高，分别命名为JsMYB108和JsMYB305。JsMYB108和JsMYB305在夜间表达量显著高于白天，与4个茉莉花萜类合成酶基因JsTPS表达特征一致。为探究JsMYB108和JsMYB305是否参与萜类香气调控，将其构建至植物表达载体pK7FWG2.0（35 S启动子，GFP报告基因）中，利用茉莉花茎段愈伤组织遗传转化体系检测其对愈伤组织中4个JsTPS基因表达水平的影响。结果显示：转化了JsMYB108和JsMYB305的愈伤组织内能检测到GFP荧光和2个MYB基因转录本;JsMYB108可显著提高JsTPS2表达水平，JsMYB305可显著提高4个JsTPS基因的表达，暗示JsMYB108和JsMYB305可能不同程度地参与了萜类合成的调控。该研究结果为今后茉莉花香气调控的深入研究提供参考。

关键词：茉莉花;MYB转录因子;TPS基因;愈伤组织;遗传转化

中图分类号：S685.16      文献标识码：A

Cloning of JsMYB108 and JsMYB305 and Analysis of Their Activation on TPS Gene in Jasminum sambac

ZHANG Yue， YUAN Yuan*， HE Xian， WANG Yuting， LYU Meiling， WU Binghua， CHEN Qingxi

College of Horticulture， Fujian Agriculture and Forestry University， Fuzhou， Fujian 350002， China

Abstract： Two MYB genes which were of high homology with fragrance regulating transcription factors in other species and predominant expressed in flower at night and were screened and cloned from the jasmine flower & leaf transcriptomes （Genebank GHOY00000000）， and the expression pattern during flower opening were detected. Results showed that the coding sequence was 864 bp and 588 bp in length， respectively， and encoded 287 and 195 amino acid residues respectively. They were of homology to Arabidopsis thaliana AtMYB108 and AtMYB24， and had the highest similarity to MYB108-like protein and MYB305-like protein in Olea Europaea var. sylvestris， and was named JsMYB108 and JsMYB305， respectively. The expression level of JsMYB108 and JsMYB305 was significantly higher at night than at day， which was consistent with the four JsTPSs expression characteristics. To investigate whether JsMYB108 and JsMYB305 were involved in the regulation of terpene synthesis， they were constructed into the plant expression vector pK7FWG2.0 （35 S promoter with GFP reporter） and transformed to jasmine callus to detect the effect on the expressions of four JsTPS genes. The results showed that GFP fluorescence and transcripts of JsMYB108 and JsMYB305 could be detected in the transformed callus. Furthermore， JsMYB108 could significantly enhance the expression of JsTPS2， while JsMYB305 could significantly increase the expression level of four JsTPS genes， suggesting that JsMYB108 and JsMYB305 might be involved in the regulation of terpene synthesis. The results of the study would lay a foundation for further studies on the regulation of jasmine fragrance.

Keywords： Jasminum sambac; MYB transcript factor; TPS gene; callus; genetic transformation

DOI： 10.3969/j.issn.1000-2561.2021.06.005

茉莉花[Jasminum sambac （L.） Aiton]为木犀科（Oleaceae）素馨属（Jasminum）常绿灌木，广泛应用于茉莉花茶窨制、香料香精、化妆品、药品、食品和园林应用等方面，是著名的芳香植物和香料作物[1]。我国茉莉鲜花基本上用于花茶生产，优质茉莉花茶每千克原料需配1.28 kg茉莉花[2]，且不同级别的茉莉花茶对其香气要求特别严格[3]。对于茉莉花茶窨茶工艺的探索以及花茶品质的研究均表明茉莉花的香气在花茶的经济和文化中起着重要作用[4-6]。……