陈峥 刘波

摘 要：芽胞杆菌是一类重要的生防微生物，能产生多样的抗菌物质。为了挖掘产喹啉类抑菌物质的菌株资源，以56个芽胞杆菌菌株为试验材料，运用液相色谱四级杆飞行时间质谱（LC-QTOF-MS）技术对其发酵产物进行检测，对质谱数据进行分子特征提取和Metlin谱库比对。结果表明：筛选获得6株产羟基喹啉基乙酮（Quinacetol）的芽胞杆菌，分别是土壤芽胞杆菌FJAT14232（Bacillus soli）、枯草芽胞杆菌FJAT8784（Bacillus subtilis）、波茨坦短芽胞杆菌FJAT10006（Brevibacillus borstelensis）、浸麻类芽胞杆菌FJAT14204（Paenibacillus macerans）、嗜碱芽胞杆菌FJAT0014（Bacillus alcalophilus）和米氏解硫胺素芽胞杆菌FJAT14205（Aneurinibacillus migulanus）。6株芽胞杆菌中的羟基喹啉基乙酮占各自总代谢物的相对含量均高于1.17%，且与Metlin谱库匹配得分均大于95.35。其中，土壤芽胞杆菌FJAT14232（Bacillus soli）中的羥基喹啉基乙酮相对含量最高，占其总代谢物相对含量的1.68%。嗜碱芽胞杆菌FJAT10014（Bacillus alcalophilus）的谱库检索得分最高，为99.13。本研究为微生物来源的喹啉类抑菌剂的开发与利用提供菌种资源和数据支持。

关键词：羟基喹啉基乙酮;喹啉类;芽胞杆菌;抗菌化合物;液相色谱四级杆飞行时间质谱

中图分类号：S 476.1   文献标志码：A   文章编号：0253-2301（2021）04-0009-07

DOI： 10.13651/j.cnki.fjnykj.2021.04.002

Screening and Analysis of Bacillus Producing Quinacetol

CHEN Zheng， Liu Bo*

（Institute of Agricultural Biological Resources， Fujian Academy ofAgricultural Sciences， Fuzhou， Fujian 350003， China）

Abstract： Bacillus is an important biocontrol microorganism which can produce a variety of antimicrobial substances. In order to mine the strain resources producing the antibacterial substance quinolines， a total of 56 Bacillus strains were used as the experimental materials and their fermentation products were detected by the liquid chromatography-quadrupole time of flight-mass spectrometry （LC-QTOF-MS）， and the molecular characteristics were extracted from the mass spectrometric data and compared with the Metlin library. The results showed that， 6 strains of Bacillus producing quinacetol were screened and obtained， including Bacillus soli FJAT14232， Bacillus subtilis FJAT8784， Brevibacillus borstelensis FJAT10006， Paenibacillus macerans FJAT14204， Bacillus alcalophilus FJAT10014， Aneurinibacillus migulanus FJAT14205. The relative contents of quinacetol in the total metabolites of the six Bacillus strains were all higher than 1.17%， and the matching scores with Metlin library were all greater than 95.35. Among them， the relative content of quinacetol in Bacillus soli FJAT14232 was the highest， accounting for 1.68% of the relative content of its total metabolite， and Bacillus alcalophilus FJAT10014 had the highest score of 99.13 by library searching. This study could provide the strain resources and data support for the development and utilization of the quinoline bacteriostatic agent from microorganisms.

Key words： Quinacetol; Quinolines; Bacillus; Antimicrobial compound; Liquid chromatography-quadrupole time of flight-mass spectrometry

羟基喹啉基乙酮（Quinacetol），又名5乙酰基8羟基喹啉、1（8羟基5喹啉）乙酮，CAS号为2598314，分子式是C11H9NO2，精确分子量为187.0630。羟基喹啉基乙酮具杀真菌活性，广泛应用于杀菌剂中。羟基喹啉基乙酮的化学结构特征是含有喹啉（C9H7N）骨架，属于喹啉类化合物。喹啉类化合物是含氮杂环中一类非常重要化合物，具有多种优良的药理作用和生物活性，应用于医药和农业的多个领域[1-2]。在医药行业中，喹啉类化合物用于疟疾、溃疡、癌症、HIV病毒和精神分裂等疾病的预防与治疗