于现花 刘军玲 金传山 张亚中 栗进才

摘 要 目的：建立同時测定西洋参破壁饮片中8种人参皂苷类成分含量的方法。方法：采用高效液相色谱-二极管阵列检测器法（HPLC-DAD）测定西洋参破壁饮片中人参皂苷Rg1、Re、Rb1、Rc、Ro、Rb2、Rb3、Rd的含量。色谱柱为Agilent5 TC-C18柱;流动相为乙腈-0.2%磷酸水溶液（梯度洗脱）;柱温为30 ℃;流速为1.0 mL/min;检测波长为203 nm;进样量为10 μL。以人参皂苷Re和人参皂苷Rb2为参照物，采用双标线性校正法预测其余6种成分的保留时间以对其进行定性，并比较该方法与相对保留时间法的差异;以人参皂苷Re为参照物，采用相对校正因子法对上述成分进行定量，并与外标法定量结果进行比较。结果：西洋参破壁饮片中人参皂苷Rg1、Re、Rb1、Rc、Ro、Rb2、Rb3、Rd的含量分别为10.59～12.78、2.160～2.768、27.492～38.880、3.154～4.018、3.368～4.080、0.343～0.755、0.961～1.415、5.857～6.923 mg/g。采用双标线性校正法预测成分保留时间的准确性更高，且预测保留时间的绝对偏差均低于相对保留时间法;采用相对校正因子法和外标法的定量结果差异不大，相对误差（RE）均小于3%。结论：所建立的双标多测法可同时对西洋参破壁饮片中8种人参皂苷类成分进行准确定性和定量。

关键词 西洋参破壁饮片;人参皂苷;双标多测法;双标线性校正法;相对校正因子法;高效液相色谱

ABSTRACT   OBJECTIVE： To establish the method for simultaneous determination of 8 kinds of ginsenosides in Panax quinquefolium broken pieces. METHODS： HPLC-DAD method was used to determine the contents of ginsenoside Rg1， Re， Rb1， Rc， Ro， Rb2， Rb3， Rd in P. quinquefolium broken pieces. The determination was performed on Agilent5 TC-C18 column with mobile phase consisted of acetonitrile-0.2% phosphoric acid water solution （gradient elution） at the flow rate of 1.0 mL/min. The column temperature was set at 30 ℃. The detection wavelength was set at 203 nm， and sample size was 10 μL. Ginsenoside Re and ginsenoside Rb2 were used as control， liner calibration with two-reference substances correction was used to predict the retention time of other 6 components， and was compared with the relative retention time method. Using ginsenoside Re as control， above components were quantified by the relative correction factor method， and the results were compared with the external standard method. RESULTS： The contents of ginsenoside Rg1， Re， Rb1， Rc， Ro， Rb2， Rb3， Rd were 10.59-12.78， 2.160-2.768， 27.492-38.880， 3.154-4.018， 3.368-4.080， 0.343-0.755， 0.961-1.415， 5.857-6.923 mg/g. The accuracy of two-reference substances linear correction method to predict the retention time of components was higher， and the absolute deviation of the predicted retention time was lower than that of the relative retention time method. There was no significant difference between the relative correction factor method and the external standard method， and relative error was <3%. CONCLUSIONS： Established two-reference substances for determination of multiple components can be used for qualitative and quantitative analysis of 8 kinds of ginsensides in P. quinquefolium broken pieces simultaneously and accurately.

KEYWORDS   Panax quinquefolium broken pieces; Ginsenoside; Two-reference substances for determination of multiple components; Two-reference substances linear correction method; Relative correction factor method; HPLC

西洋参为五加科植物西洋参Panax quinquefolium L.的干燥根，原生长于北美洲的东部，我国于17世纪开始引进种植，已有300多年的应用历史[1-3]。传统中医理论认为，西洋参性凉而补，具有补气养阴、清热生津的功效，主要用于阴虚燥咳、劳嗽咳血等[1]。现代药理学研究表明，西洋参具有抗肿瘤、免疫调节、改善心血管系统疾病等作用[4-5]。……