冯敬骞 李姜言 宋剑锋 王思为 毛碧增

摘 要 目的：綜合评价不同产地衢枳壳药材的质量。方法：采用高效液相色谱法（HPLC）测定衢枳壳药材中圣草次苷、芸香柚皮苷、柚皮苷、柚皮素、橙皮苷、新橙皮苷的含量;采用紫外-可见分光光度法测定衢枳壳药材中总黄酮的含量;采用2020年版《中国药典》（四部）附录2204 挥发油测定法甲法测定衢枳壳药材中挥发油的含量。以14批不同产地衢枳壳药材中上述6个黄酮类成分、总黄酮及挥发油的含量为指标，采用熵权TOPSIS法综合评价衢枳壳药材的整体质量。结果：14批不同产地衢枳壳药材中圣草次苷、芸香柚皮苷、柚皮苷、柚皮素、橙皮苷、新橙皮苷、总黄酮的含量分别为2.530 4～2.915 1、4.273 7～5.313 8、34.715 8～36.695 8、3.061 1～4.456 0、4.560 9～5.773 5、45.153 6～47.019 7、145.480 1～157.022 5 mg/g，挥发油的含量为0.034～0.040 mL/g。熵权TOPSIS法分析结果显示，上述各指标权重分别为0.109 0、0.140 7、0.148 3、0.125 5、0.067 0、0.139 0、0.124 2、0.146 3;14批不同产地衢枳壳药材的平均相对贴近度分别为0.809 0、0.711 9、0.807 0、0.681 5、0.447 0、0.363 1、0.439 8、0.349 6、0.449 6、0.376 5、0.486 6、0.458 7、0.282 0、0.167 0。质量排名前4位的衢枳壳药材分别为常山县大宝山、常山县太公山、常山县鹭山和常山县小翠山产样品。结论：采用熵权TOPSIS法可对衢枳壳药材质量进行综合评价;常山县所产衢枳壳药材质量较优。

关键词 衢枳壳;含量测定;熵权TOPSIS法;质量评价

ABSTRACT   OBJECTIVE： To evaluate the quality of Citrus Paradisi from different producing areas comprehensively. METHODS： The contents of eriocitrin， narirutin， naringin， naringenin， hesperidin and neohesperidin in Citrus Paradisi were determined by HPLC method; the contents of total flavonoids were determined by UV spectrophotometry; the contents of volatile oil were determined by volatile oil measurement method A stated in appendix 2204 of Chinese Pharmacopoeia （part Ⅳ）. Using the contents of above 6 kinds of flavonoids componets， total flavonoids and volatile oil in 14 batches of Citrus Paradisi from different habitats as indexes， the overall quality of Citrus Paradisi was evaluated by using entropy weight TOPSIS method. RESULTS： The contents of eriocitrin， narirutin， naringin， naringenin， hesperidin， neohesperidin， total flavonoids in 14 batches of Citrus Paradisi from different producing areas were 2.530 4-2.915 1， 4.273 7-5.313 8， 34.715 8-36.695 8， 3.061 1-4.456 0， 4.560 9-5.773 5， 45.153 6-47.019 7， 145.480 1-157.022 5 mg/g， and that of volatile oil was 0.034-0.040 mL/g， respectively. The entropy weight TOPSIS analysis showed that the weights of them were respectively 0.109 0， 0.140 7， 0.148 3， 0.125 5， 0.067 0， 0.139 0， 0.124 2， 0.146 3. The relative closeness degree of 14 batches of samples from different areas were respectively 0.809 0， 0.711 9， 0.807 0， 0.681 5， 0.447 0， 0.363 1， 0.439 8， 0.349 6， 0.449 6， 0.376 5， 0.486 6， 0.458 7， 0.282 0， 0.167 0. Top 4 samples in the quality evaluation were collected from Dabaoshan， Taigongshan， Lushan and Xiaocuishan of Changshan county. CONCLUSIONS： The entropy weight TOPSIS method can be used for the comprehensive quality evaluation of Citrus Paradisi. The quality of Citrus Paradisi from Changshan county is better than that of samples from other areas.

KEY WORDS   Citrus Paradisi; Content determination; Entropy weight TOPSIS method; Quality evaluation

衢枳壳主产于浙江省衢州市，为芸香科植物常山胡柚Citrus changshan-huyou Y.B.Chang的未成熟果实，一般于每年6～7月采收（此时果实直径约5～7 cm）[1]，收载于《浙江省中药炮制规范》（2015年版）[2]。衢枳壳具有理气宽中、行滞消胀等功效，临床上常将其用于治疗胸胁气滞、胀满疼痛、食积不化、痰饮内停、脏器下垂等[2]。现代药理研究还发现，衢枳壳具有抗氧化、抗菌、抗病毒、降血脂和降血糖等药理作用[3-7]。……