张锋涛 张成 廖文敏 付岗 张重远 侯巨梅 李培征 刘铜

摘    要：通过形态学和分子生物学相结合的方法，明确木霉菌株SX005的分类地位，并以该菌株的分生孢子粉为原料，分别与氨基寡糖素、矿源黄腐酸钾、壳寡糖、海藻精、FUNA-801进行复配，通过盆栽试验测定其复合制剂对黄瓜和辣椒苗生长的影响。结果表明，木霉菌株SX005为钩状木霉菌;其5种木霉复配制剂均能促进黄瓜和辣椒苗地上部分生长，且比单独施用木霉菌效果更好，其中氨基寡糖素与木霉菌复配的制剂对2种作物的地上部分生长促进效果最好，使黄瓜株高提高了172%，辣椒株高提高了59.56%。海藻精或壳寡糖与木霉菌复配的制剂可显著增加黄瓜根质量，氨基寡糖素或矿源黄腐酸钾与木霉菌复配制剂可显著增加辣椒根质量。本研究将为钩状木霉SX005复合制剂应用提供技术支撑。

关键词：黄瓜;辣椒;钩状木霉;SX005;促生

中图分类号：S642.2+S641.3 文献标志码：A 文章编号：1673-2871（2021）02-023-07

Identification of Trichoderma hamatum SX005 and effects of its six compound preparation on the growth of cucumber and pepper seedlings

ZHANG Fengtao1， ZHANG Cheng1， LIAO Wenmin2， FU Gang3， ZHANG Chongyuan1， HOU Jumei1， LI Peizheng1， LIU Tong1

（1. Engineering Center of Agricultural Microbial Preparation Research and Development of Hainan （Hainan University）， Haikou 570228， Hainan， China;2. Haikou R & D Center of Guizhou Yibaiyi Biotechnology Co.， Ltd， Haikou 570100，Hainan， China;3. Institute of Plant Protection Research， Guangxi Academy of Agricultural Sciences， Nanning 530007，Guangxi， China）

Abstract： The classification status of Trichoderma strain SX005 was clarified through morphological characteristics combined with molecular biology ， and the growth-promoting function of its compound preparation on cucumber and pepper seedlings was evaluated by pot experiments with T. hamatum SX005 conidia as raw materials， compounded with amino oligosaccharide， potassium fulvic acid， chitooligosaccharide， seaweed extract and FUNA-801. Results showed that the Trichoderma strain SX005 was identified as T. hamatum based on the characteristics of morphology and molecular biology. The five Trichoderma preparations could promote the growth of cucumber and pepper seedlings， and the effect was better than that of Trichoderma alone. The mixure of amino oligosaccharide and Trichoderma had the best promotion effect on the above-ground growth of the two crops， the plant height of cucumber and pepper increased by 172% and 59.56% respectively. The mixture of seaweed extract or chitosan and Trichoderma could significantly increase the root weight of cucumber， aminooligosaccharide or mineral potassium fulvic acid combined with Trichoderma could significantly increase the root weight of pepper. This study will provide technical support for the application of T. hamatum SX005 compound.

Key words： Cucumber; Pepper; Trichoderma hamatum; SX005; Promoting growth

黃瓜和辣椒是我国重的要经济作物，近年来，在设施栽培中过量施用化学肥料引起了土壤酸化、板结、重金属污染和次生盐渍化等问题，降低土壤肥力的同时污染生态环境[1-2]。而在肥料中添加含有特定功能的菌剂，制成微生物肥料可以抑制作物对重金属、农药的吸收，净化和修复土壤，提高肥料利用率[3]。已有研究表明，在黄瓜和辣椒种植中施用微生物菌剂能显著提高产量、降低作物发病率[4-6]。木霉菌（Trichoderma spp.）是广泛存在于土壤、根围、叶围等自然生态环境中的一种真菌，它可以通过竞争、拮抗、重寄生和分泌次生代谢产物等方式抑制植物病原菌的生长，同时可以促进植物对养分的吸收利用，提高作物产量和品质，在现代绿色农业生产中被广泛使用[7-11]。……