廖锦钰 黄莉萍 张战泓 张德咏 谭新球 刘勇 史晓斌

摘要 ：番茄褪绿病毒Tomato chlorosis virus（ToCV）是一种由烟粉虱Bemisia tabaci传播的正义单链RNA病毒，在田间常与番茄黄化曲叶病毒Tomato yellow leaf curl virus（TYLCV）复合侵染而造成番茄生产上重大的经济损失。为了明确ToCV与TYLCV的复合侵染对烟粉虱传播ToCV所造成的影响，本文采用RT-PCR以及qRT-PCR检测了复合侵染的番茄对烟粉虱获取和传播ToCV的影响。研究表明，烟粉虱取食复合侵染的番茄后对ToCV的传播效率显著提高，仅25头烟粉虱的传毒率即可达到100%，ToCV在烟粉虱以及番茄体内的累积量均显著提高。说明这种复合侵染促进了烟粉虱对ToCV的传播，在田间应当及时防控烟粉虱，警惕病毒与烟粉虱的蔓延。

关键词 ：番茄褪绿病毒; 番茄黄化曲叶病毒; 烟粉虱; 复合侵染

中图分类号：

S 436.412.11

文献标识码： A

DOI： 10.16688/j.zwbh.2020063

Influence of co-infection of Tomato chlorosis virus and Tomato yellow

leaf curl virus on the transmission of Tomato chlorosis virus

LIAO Jinyu1，2， HUANG Liping1，2， ZHANG Zhanhong3， ZHANG Deyong2， TAN Xinqiu2， LIU Yong2*， SHI Xiaobin1，2*

（1. Subcollege of Longping， Graduate School of Hunan University， Changsha 410128， China;

2. Hunan Plant Protection Institute， Hunan Academy of Agricultural Sciences， Changsha 410125， China;

3. Hunan Vegetable Institute， Hunan Academy of Agricultural Sciences， Changsha 410125， China）

Abstract

Tomato chlorosis virus （ToCV） is a positive single-stranded RNA virus transmitted by Bemisia tabaci， which is usually co-infected with Tomato yellow leaf curl virus （TYLCV） in the field and causes significant economic losses in tomato production. In order to clarify the influence of co-infection of ToCV and TYLCV on the transmission of ToCV， RT-PCR and real-time quantitative PCR were used to detect the effect of co-infection on the virus acquisition and transmission of ToCV by B.tabaci. The results showed that the ToCV transmission efficiency was significantly improved after co-infection. Only 25 B.tabaci individuals could cause a 100% transmission rate， and the quantities of ToCV in B.tabaci and tomato were significantly increased. This co-infection promoted the spread of ToCV by B.tabaci. The whitefly should be prevented timely， and the spread of virus and whitefly should be highly regarded in the field.

Key words

Tomato chlorosis virus; Tomato yellow leaf curl virus; Bemisia tabaci; co-infection

番茄褪绿病毒Tomato chlorosis virus （ToCV），隶属于长线性病毒科Closteroviridae，毛形病毒属Crinivirus，是一种由烟粉虱Bemisia tabaci传播的半持久性RNA病毒，于2004年在我国台湾首次报道[1]。番茄黄化曲叶病毒Tomato yellow leaf curl virus （TYLCV），隶属于双生病毒科Geminiviridae，菜豆金色花叶病毒属Begomovirus，是一种单链环状DNA植物病毒，田间依靠烟粉虱以持久性非增殖方式传播，于2006年在我国上海首次报道[2]。ToCV与TYLCV的发病时期和所需的环境条件大致相同，傳播媒介相似，发病症状均表现为叶片黄化，其主要区别在于ToCV通常表现为老叶先发病，叶片脉间黄化褪绿，同时变厚变脆且边缘向内卷曲;而TYLCV通常表现为新叶先发病，叶缘逐渐黄化，同时变小皱缩且向上卷曲[34]。此外，ToCV与TYLCV发病的潜伏期差异较大，ToCV的潜伏期较长，一般需要3～4周才能表现出症状，而TYLCV的潜伏期较短，一般7～10 d就开始表现发病症状[34]。……