谢殿杰 张蕾 程云霞 江幸福

摘要 ：为了明确黏虫MagR、Cry2基因在定向行为中的作用，本研究采用基因序列分析及实时荧光定量技术，研究了黏虫MagR、Cry2基因的基因序列特征及其表达模式。结果表明，黏虫MagR基因编码131个氨基酸，蛋白分子量为14.17 kD，等电点为9.3，具有多个铁硫簇结合位点;黏虫Cry2基因编码757个氨基酸，蛋白分子量为87.04 kD，等电点为8.73。羽化后不同日龄迁飞型黏虫成虫各部位Cry2基因表达量存在显著差异。羽化后3 d成虫头部和胸部Cry2基因表达量显著高于其他日龄。迁飞型黏虫成虫羽化后不同日龄各部位MagR的表达量具有显著性差异;5 d时各部位的表达量达到最高，各日龄头部和胸部的表达量均显著高于腹部。

关键词 ：黏虫; 时空表达; 定向; Cry2; MagR

中图分类号：

S 433

文献标识码： A

DOI： 10.16688/j.zwbh.2020035

Temporal and spatial expression of MagR and Cry2 genes in Mythimna separata

XIE Dianjie， ZHANG Lei， CHENG Yunxia， JIANG Xingfu*

（State Key Laboratory for Biology of Plant Diseases and Insect Pests， Institute of Plant Protection， Chinese Academy of Agricultural Sciences， Beijing 100193， China）

Abstract

In order to clarify the role of MagR and Cry2 genes in the orientation behavior of Mythimna separata， the gene sequence analysis and qRT-PCR were performed to study the gene sequence characteristics and expression patterns of MagR and Cry2 genes. The results showed that MagR gene encoded 131 amino acids， with a molecular weight of 14.17 kD， an isoelectric point of 9.3 and many iron sulfur cluster binding sites. The Cry2 gene encoded 757 amino acids， with a molecular weight of 87.04 kD and an isoelectric point of 8.73. After eclosion， there were significant differences of Cry2 gene expression in different parts of M.separata at different day-ages. The Cry2 gene expression in the head and thorax of 3-day-old adults was significantly higher than that in the adults of other day-ages. Meanwhile， after eclosion， there were also significant differences of MagR expression in different parts of M.separata at different day-ages. At 5-day-age， the expression level of each part reached the highest， and the expression levels in the head and thorax at each age were significantly higher than those in the abdomen.

Key words

Mythimna separata; temporal and spatial expression; orientation; Cry2; MagR

黏蟲Mythimna separata属鳞翅目，夜蛾科，为典型的远距离季节性迁飞害虫。黏虫迁飞距离最远可达1 400 km[1]，主要在亚洲、大洋洲发生为害。在我国，除新疆外，其他地区均发现黏虫为害[2]。黏虫寄主种类多，为害范围广，对我国水稻、小麦和玉米三大主粮作物造成严重危害，同时也会对其他禾本科作物、牧草、经济作物和油料作物造成不同程度的损失。在我国，黏虫每年有多次大范围的往返迁飞为害活动，迁飞是其造成危害的主要方式。

“起飞、运转、降落”为昆虫迁飞的三个过程[3]。昆虫起飞后可以借助内部和外部的各种信号主动调整自身姿态及空间位置从而准确到达目的地的现象称为昆虫的定向[4]。目前，对昆虫定向行为有较多研究的主要有蜜蜂、蚂蚁、果蝇等近距离飞行昆虫，以及蝴蝶、蛾类、蝗虫等远距离迁飞昆虫。……