陈晨 李永圆 王海霞 于洋 李正

摘要 目的：納米粒子特有的小尺寸效应和表面效应使得纳米银粒子具有广谱抗菌活性。但纳米银粒子浓度过高可能会对其他细胞或器官等造成毒性或损伤，所以为了更好地发挥抗菌活性，对纳米银粒子进行快速、准确的分析是极为必要的。方法：通过对纳米银粒子吸附方法和纸基芯片的优化和筛选，测定纳米银粒子的激光诱导击穿光谱图。通过特征峰位与标准谱图库进行比对对纳米银粒子进行定性分析，通过特征光谱强度与纳米银浓度的线性回归关系建立纳米银粒子分析的定量模型。结果：通过重复3次“浸渍-干燥”工艺，定量滤纸表面吸附的纳米银粒子在328 nm、338 nm、520 nm、546 nm和827 nm处显示出银的特征峰。在312.5～5 000 μg/mL浓度范围内，520 nm波长处纳米银的特征峰强与浓度呈良好线性关系（r=0.998 8）。结论：激光诱导击穿光谱分析技术可用于纳米银定性定量分析，分析方法准确，灵敏度高，且检测过程方便便捷，易于实际推广。

关键词 激光诱导击穿光谱;纳米银;定量分析;定性分析

Development of Qualitative and Quantitative Analysis Method of Silver Nanoparticles Based on Laser Induced Breakdown Spectroscopy

CHEN Chen1，LI Yongyuan1，WANG Haixia1，2，3，YU Yang1，2，3，LI Zheng1，2，3

（1 College of Pharmaceutical Engineering of Traditional Chinese Medicine，Tianjin University of Traditional Chinese Medicine，Tianjin 301617，China; 2 State Key Laboratory of Component-based Chinese Medicine，Tianjin University of Traditional Chinese Medicine，Tianjin 301617，China; 3 Haihe Laboratory of Modern Chinese Medicine，Tianjin 301617，China）

Abstract Objective：The unique small size effect and surface effect of nanoparticles make silver nanoparticles have broad-spectrum antibacterial activity.However，too high concentration of silver nanoparticles may cause toxicity or damage to other cells or organs.Therefore，in order to better exert antibacterial activity，it is extremely necessary to analyze silver nanoparticles quickly and accurately.Methods：By optimizing and screening the nano-silver particle adsorption method and paper-based chip，the laser-induced breakdown spectra of nano-silver particles were measured.The qualitative analysis of nano-silver particles was carried out by comparing the characteristic peak position with the standard spectral library，and the quantitative model of nano-silver particle analysis was established by the linear regression relationship between the characteristic spectral intensity and the concentration of nano-silver.Results：By repeating the “dipping-drying” process three times，the nano-silver particles adsorbed on the surface of the quantitative filter paper showed characteristic peaks of silver at 328 nm，338 nm，520 nm，546 nm and 827 nm.In the concentration range of 312.5～5 000 μg/mL，the characteristic peak intensity of nano-silver at the wavelength of 520 nm had a good linear relationship with the concentration （r=0.998 8）.Conclusion：The LIBS analysis technology can be used for qualitative and quantitative analysis of nano-silver.The analysis method is accurate and sensitive，and the detection process is convenient and convenient，which can be easily used in practice.

Keywords Laser induced breakdown spectroscopy; Nano-silver particles; Quantitative analysis; Qualitative analysis

中图分类号：R284文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.23.004

纳米银颗粒具有广谱抗菌活性，对多种革兰阳性、革兰阴性细菌具有抑菌和杀菌作用[1]。据报道，纳米银粒子主要的抑菌机制是基于纳米银表面的阳离子与细菌蛋白中的硫醇基团间的特异性结合能力，在细菌表面黏附积累并穿透细胞膜，导致细胞膜和细胞成分被破坏，从而抑制或阻止细菌生长和细胞分裂，最终导致细菌死亡[2]。这种抑菌活性与纳米银颗粒的大小、形状和浓度紧密相关，如果浓度过高，会对其他细胞或器官等造成毒性或严重损伤[3]。……