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不同品种及产地木耳中多糖含量比较研究

2021-02-03李振陈结梅刘贤钊谭丽容孙红梅

农学学报 2021年3期
关键词:黑木耳

李振 陈结梅 刘贤钊 谭丽容 孙红梅

摘要:建立木耳多糖新的测定方法,比较不同品种及产地木耳多糖含量的差异,为木耳的质量评价提供依据。采用蒽酮-硫酸法分别测定黑木耳、毛木耳不同产地的多糖含量,并进行方法学考察。葡聚糖在3.27~23.61μg/mL范围内,其浓度与对应的吸光度呈良好的线性关系(R2=0.9993),平均回收率为101.2%,RSD为3.5%(n=9)。浙江江山、辽宁本溪、福建漳州产黑木耳中多糖含量分别为4.1%、3.0%和3.5%;浙江江山和江苏徐州产毛木耳中多糖含量分别为4.1%和3.6%。蒽酮-硫酸法简便可行、重复性好,适用于木耳中多糖含量的测定。不同产地的黑木耳多糖含量有一定的差异,不同产地的毛木耳多糖含量差异不明显,同产地或地域位置相近的不同产地黑木耳与毛木耳品种间的多糖含量无明显差异。

关键词:黑木耳;毛木耳;多糖;蒽酮-硫酸法;产地

中图分类号:S184文献标志码:A论文编号:cjas2020-0155

Auricularia of Different Varieties and Habitats: A Comparative Study on the Polysaccharides Content

Li Zhen1, Chen Jiemei1, Liu Xianzhao1, Tan Lirong2, Sun Hongmei1

(1Infinitus (China) Co., Ltd., Jiangmen 529100, Guangdong, China; 2Hongzhengdao (China) Traditional Chinese Medicine Research Company Ltd., Guangzhou 510665, Guangdong, China)

Abstract: The paper aims to establish a method for the determination of polysaccharides in Auricularia and compare the polysaccharide content of Auricularia in different varieties and regions. Anthrone-sulfuric acid method was used to determine the content of polysaccharides in Auricularia auricula and Auricularia polytricha from different habitats, and the methodological investigation was conducted. The results showed that: the concentration of dextran had a good linear relationship with its absorbance in the range of 3.27-23.61μg/mL(R2=0.9993), with an average recovery rate of 101.2% and RSD of 3.5% (n=9). The polysaccharides content of A. auricula from Jiangshan, Benxi and Zhangzhou was 4.1%, 3.0% and 3.5%, respectively; the polysaccharides content of A. polytricha from Jiangshan and Xuzhou was 4.1% and 3.6%, respectively. The method is suitable for the determination of polysaccharides in Auricularia. There was certain difference in the polysaccharides content of A. auricula from different habitats, but not for the A. polytricha. The overall difference in the polysaccharides content between the two varieties was not obvious.

Keywords: Auricularia auricular; Auricularia polytricha; Polysaccharides; Anthrone-sulfuric Acid Method; Region

0引言

木耳(Auricularia)屬担子菌纲木耳目木耳科,是一种主产于中国的大型食用真菌,营养价值丰富[1],有“素中之荤”的美誉。木耳的种类多样,常见的有黑木耳(Auriculariaauricula)和毛木耳(Auricularia polytricha),也是目前在黑龙江、辽宁、吉林、福建、江苏、浙江、湖北、四川、云南、广西等地广泛栽培种类[2-3]。木耳多糖是木耳子实体的重要活性物质,具有抗氧化[4-5]、抗辐射[6]、抗病毒[7]、抗肿瘤[8-9]、降血糖[10-11]、降血脂[12-13]、增强机体免疫[14]等多种有益于人体健康的功能作用。作为原料,木耳已被应用于功能食品、保健品及药品等领域。

目前,行业内尚未制定木耳多糖含量检测方法的国家标……

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