苏振华

摘要：目的  探讨乙肝病毒标记物阳性表型血清标本在乙型肝炎病毒诊断中的应用。方法  选取2018年5月～2019年5月我院收治的乙肝病毒阳性患者100例作为实验组，另选取同期我院健康体检者100例作为对照组，采用酶联免疫吸附法与PCR荧光定量法对两组乙肝病毒标记物表型血清指标进行检测，比较兩组阳性反应组合反应阳性率、HBV-DNA水平。结果  通过酶联免疫吸附法检验，在Presl、HBsAb、HBsAg、HBeAg、HBeAb、HBcAb-IgM、HBcAb-IgG等指标中可表现出11种阳性反应组合，其中HBeAb、HBsAg、HBcAb-IgG阳性反应组合阳性率最高，其次为Presl、HBeAg、HBsAg、HBcAb-IgG阳性反应组合;实验组HBV-DNA水平为（4.70±1.30）×104，对照组HBV-DNA水平为（0.80±0.10）×104，实验组高于对照组，差异有统计学意义（P<0.05）。结论  乙肝病毒标记物表型血清标本检测在乙型肝炎病毒中诊断的价值较高，可为临床诊断提供科学依据。

关键词：乙型肝炎病毒;乙肝病毒标记物表型;血清标本

中图分类号：R737.33                               文献标识码：A                                  DOI：10.3969/j.issn.1006-1959.2020.04.061

文章编号：1006-1959（2020）04-0179-02

Abstract：Objective  To investigate the application of hepatitis B virus marker positive phenotype serum samples in the diagnosis of hepatitis B virus.Methods  100 patients with HBV-positive patients admitted to our hospital from May 2018 to May 2019 were selected as the experimental group，and another 100 healthy subjects from our hospital during the same period were selected as the control group.ELISA and PCR fluorescence quantitative methods were used the serum index of HBV marker phenotype was detected in the two groups，and the positive rate of combined positive reactions and HBV-DNA levels were compared between the two groups.Results  By enzyme-linked immunosorbent assay，11 positive reaction combinations can be shown in Presl， HBsAb， HBsAg， HBeAg， HBeAb， HBcAb-IgM， HBcAb-IgG and other indicators， among which HBeAb， HBsAg， HBcAb-IgG positive combination positive highest，followed by Presl， HBeAg， HBsAg，and HBcAb-IgG positive reaction combinations;HBV-DNA level in the experimental group was（4.70±1.30）×104，and HBV-DNA level in the control group was（0.80±0.10）×104，the experimental group was higher than the control group，the difference was statistically significant（P<0.05）.Conclusion  The detection of HBV marker phenotype serum samples is of higher diagnostic value in HBV，and it can provide scientific basis for clinical diagnosis.

Key words：Hepatitis B virus;HBV marker phenotype;Serum specimen

乙型病毒性肝炎（viral hepatitis type B）属于一种临床常见的肝脏炎性病变，严重威胁人们的健康，该病在全世界范围内都有着较高的发病率，并且该病辐射年龄较广，患者在发病后如果没有采取及时有效治疗措施，可能会导致疾病进一步恶化，进而导致肝癌与肝硬化发生。在临床诊断中一般予以肝功能检查，并且将血清病原学指标作为诊断依据，但依靠阳性表型指标无法准确反映患者体内乙肝病毒存在情况，同时也无法对疾病进展进行有效监测，临床误诊、漏诊率较高。患者常在出现确切症状后才会参与病理诊断，延误最佳治疗时机，预后较差，本研究主要探讨乙肝病毒标记物阳性表型血清标本乙型肝炎病毒HBV-DNA临床检验，现报道如下。……