甘国兴 吴晓芳 冯伟勋 邝卫红 李劲平 谢保平 张毅之

[摘要] 目的 研究五磨飲对脾虚气滞证功能性消化不良（FD）大鼠的干预作用。 方法 70只雌性SD大鼠随机选出10只作为正常组，其余采用“慢性束缚+游泳+饮食不节”复合因素法制备脾虚气滞证FD模型。48只造模成功大鼠随机分为模型组、多潘立酮组、柴芍六君子汤组、五磨饮高剂量组、五磨饮中剂量组、五磨饮低剂量组，每组8只。除正常组及模型组给予等体积纯净水外，其余大鼠灌胃给药干预14 d。记录大鼠食量和体质量，测定小肠推进率，检测胃窦及下丘脑组织诱导型一氧化氮合酶（iNOS）、P物质（SP）、5-羟色胺（5-HT）表达。 结果 与模型组比较，给药第5、10、15天，正常组、各给药组大鼠食量显著增加（P < 0.05或P < 0.01）。与模型组比较，给药第5天，正常组、多潘立酮组大鼠体质量显著增加（P < 0.05或P < 0.01）;与模型组比较，给药第10、15天，正常组、各给药组大鼠体质量显著增加（P < 0.05或P < 0.01）。与模型组比较，正常组、各给药组大鼠小肠推进率显著增加（P < 0.01）。与模型组比较，各给药组、正常组大鼠胃窦组织iNOS表达显著下调（P < 0.05）;与模型组比较，多潘立酮组、五磨饮低剂量组大鼠胃窦组织SP表达显著下调（P < 0.01）;与模型组比较，五磨饮高剂量组大鼠胃窦组织5-HT表达上调（P < 0.05）。与模型组比较，正常组、多潘立酮组、柴芍六君子汤组、五磨饮中剂量组大鼠下丘脑iNOS表达显著升高（P < 0.05或P < 0.01）;与模型组比较，正常组、柴芍六君子汤组、五磨饮中剂量组大鼠下丘脑SP表达显著升高（P < 0.05或P < 0.01）。 结论 五磨饮对脾虚气滞证FD模型大鼠具有一定的疗效，调节脑-肠轴iNOS、SP、5-HT表达可能是其作用机制之一。

[关键词] 功能性消化不良;五磨饮;药效;脑肠肽

[中图分类号] R318.14          [文献标识码] A          [文章编号] 1673-7210（2019）07（a）-0020-05

Intervention effect of Wumoyin Decoction on functional dyspepsia rats of spleen deficiency and Qi stagnation syndrome

GAN Guoxing1   WU Xiaofang2   FENG Weixun1   KUANG Weihong3   LI Jingpin4   XIE Baopin5   ZHANG Yizhi1

1.Department of Pharmacy， Qingyuan Hospital of TCM， Guangdong Province， Qingyuan   511500， China; 2.School of Nursing， Qingyuan Polytechnic， Guangdong Province， Qingyuan   511510， China; 3.the First Affiliated Hospital of Guangzhou University of TCM， Guangdong Province， Guangzhou   510006， China; 4.College of Pharmacy， Central South University， Hu′nan Province， Changsha   410013， China; 5.Key Laboratory， Prevention and Treatment of Cardiovascular and Cerebrovascular Disease of Ministry of Education of Gannan Medical University， Jiangxi Province， Ganzhou   341000， China

[Abstract] Objective To study the effect of Wumoyin Decoction on FD rats of spleen deficiency and Qi stagnation syndrome. Methods Ten rats were randomly selected as normal group from 70 female SD rats， and other rats were modeled by "chronic restraint + swimming + irregular eating" compound factor method. 48 modeled rats were randomly divided into model group， Domperidone group， Chaishao Liujunzi Decoction group， Wumoyin Decoction high， middle and low dose group， 8 rats in each group. Besides normal group and model group， the other groups of rats were given the corresponding drug intervention for 14 days. The food intake and body weight were recorded， the small intestine propulsion rate was determined， the expression of iNOS， SP， 5-HT in antral tissue and hypothalamus were detected. Results Compared with model group， the food intake of rats in normal group and administration groups were increased significantly on the 5th， 10th and 15th day of administration （P < 0.05 or P < 0.01）. Compared with model group， body mass of rats in normal group and Domperidone group were increased significantly on the 5th day of administration （P < 0.05 or P < 0.01）. Compared with model group， body mass of rats in normal group and administration groups were increased significantly on the 10th and 15th day of administration （P < 0.05 or P < 0.01）. Compared with model group， the propulsive rate of small intestine in normal group and administration groups were increased significantly （P < 0.01）. The expression of iNOS in gastric antrum tissue of rats in administration groups and normal groups were significantly down-regulated compared with model group （P < 0.05）. Compared with model group， SP expression in gastric antrum tissue of Domperidone group and Wumoyin low dose group rats were significantly deed （P < 0.01）. Compared with the model group， the expression of 5-HT in gastric antrum tissue of rats in high dose group of Wumoyin was up-regulated （P < 0.05）. Compared with model group， the expression of iNOS in hypothalamus of normal group， Domperidone group， Chaishao Liujunzi Decoction group and Wumoyin medium dose group were significantly increased （P < 0.05 or P < 0.01）. Compared with model group， the expression of SP in hypothalamus of normal group， Chaishao Liujunzi Decoction group and Wumoyin medium dose group rats was significantly increased （P < 0.05 or P < 0.01）. Conclusion Wumoyin Decoction has certain curative effect on FD rats of spleen deficiency and Qi stagnation syndrome， regulating the expression of iNOS， SP and 5-HT in the brain-gut axis may be one of its mechanisms.