范作卿　邹德庆　王娜　郭光　朱琳

摘要：为了解柞蚕雄蛾营养液保肝降酶的功能，采用谷丙转氨酶活性检测与病理组织学切片观察的方法，研究柞蚕雄蛾营养液对四氯化碳中毒小鼠肝损伤的作用。结果表明：柞蚕雄蛾营养液大剂量组与小剂量组均可明显降低四氯化碳中毒小鼠血清中谷丙转氨酶（ALT）水平，治疗效果达显著水平（P<0.05），与乙肝宁具有相同作用。通过肝组织切片观察，柞蚕雄蛾营养液小剂量组可见部分肝细胞点状坏死，有轻度肝损伤，而柞蚕雄蛾营养液大剂量组小鼠肝组织结构正常，肝细胞排列规则，未见炎症。表明柞蚕雄蛾营养液能减少肝细胞变性、坏死的程度和范围，对受损伤的肝组织具有明显保护作用，且大剂量效果更佳。

关键词：柞蚕雄蛾营养液；四氯化碳；保肝；谷丙转氨酶；功能试验

中图分类号：S885.1：F307.5 文献标识号：A 文章编号：1001-4942（2018）08-0148-04

Study on Liver-Protecting Function of Tussah

Male Moth Nutrient Solution on Mice

Fan Zuoqing， Zou Deqing， Wang Na， Guo Guang， Zhu Lin

（Sericultural Research Institute of Shandong Province， Yantai 264002， China）

Abstract To understand the liver-protecting function of tussah male moth nutrient solution， the alanine transaminase （ALT） activity assay and histopathological observation were used to study the effect of tussah male moth nutrient solution on liver injury of mice induced by carbon tetrachloride poisoning. The results showed that the tussah male moth nutrition in high dose group and low dose group both could decrease ALT level in serum of poisoning mice. The therapeutic effect was significant compared with the carbon tetrachloride model group（P<0.05）. And its effect was the same as that of positive drug Yiganning. The histopathologic examination showed that there were partial necrosis of liver cells and mild liver injury in low dose group. While the liver tissue structure was normal in high dose group. The liver cells arranged regularly， and no inflammation occurred. Therefore， it indicated that the nutrient solution of tussah moth could reduce the extent of liver cell degeneration and necrosis， and had a significant protective effect on the injured liver， and high doses were better.

Keywords Tussah male moth nutrient solution； Carbon tetrachloride； Liver protection； Alanine transaminase （ALT）； Function test

我國柞蚕资源丰富，近年来对柞蚕雄蛾的综合加工利用已引起人们的高度重视，柞蚕雄蛾的相关保健品及药物已被证明具有增强机体免疫力[1]、降血脂[2]、抗肿瘤[3]等多种功能。现已开发出一些食品、保健品和医药品，取得了较大的经济效益和社会效益。例如“延生护宝液”系以雄蚕蛾为主药，配以人参、鹿茸、驴肾、仙茅、肉桂等多味中药而成，对男女肾虚引起的疾病有一定的防治作用[4]。新型中药复方制剂——柞蚕雄蛾口服液具有明显增强免疫力，降低过氧脂质（Lpo）含量的抗衰老作用[5]。柞蚕雄蛾营养液是山东省蚕业研究所研究团队选用初羽化、未交尾的柞蚕雄蛾为原料，通过酒精浸泡后低温减压抽提得到的富含生物活性的保健产品，现已完成了其提取工艺研究及成分检测[6，7]。柞蚕雄蛾营养液可明显降低由半胱氨酸所致高脂血症小鼠血清中的血脂四项水平，因而对于高脂血症有良好的治疗效果，经试验证明其有……